Methanol-soluble, β-elimination products from preparations of alginic acid hydroxamate exhibited DPPH scavenging and angiotensin converting enzyme inhibitory activities

Yuh H. Liu, Mao T. Chuang, Wen C. Hou

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The methanol-soluble sugar hydroxamic acid derivatives obtained during the preparation of alginic acid hydroxamates (AAH) were separated by BioSil-ODS HPLC column (10x250 mm) in acetonitrite: 0.05% trifluoroacetic acid, 10:90 (V/V). The absorbance at 235 nm was set for monitoring β-elimination products. Each fraction was collected and assayed for hydroxamic acid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and inhibitory activity against angiotensin converting enzyme (ACE). The fraction with higher hydroxamic acid content was further separated by Sephadex G-15 column. Fractions containing both ACE inhibitory activity and DPPH scavenging activity overlapped with fractions containing high levels of hydroxamic acid derivatives. The chromatogram showed little tailing when judged by hydroxamic-acid content but significant tailing when judged by biological activity. Each fraction from above was further separated by silica-TLC in acetonitrite: distilled water, 95:5 (V/V). It was found that a methanol soluble fraction prepared from alginic acid hydroxamates (AAH) exhibited DPPH scavenging activity.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalBotanical Studies
Volume48
Issue number2
Publication statusPublished - Apr 2007

Fingerprint

hydroxamic acids
peptidyl-dipeptidase A
alginates
methanol
silica
absorbance
bioactive properties
2,2-diphenyl-1-picrylhydrazyl
sugars
monitoring
acids
water

Keywords

  • β-elimination
  • Alginic acid hydroxamates
  • Angiotensin converting enzyme (ACE)
  • DPPH

ASJC Scopus subject areas

  • Plant Science

Cite this

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title = "Methanol-soluble, β-elimination products from preparations of alginic acid hydroxamate exhibited DPPH scavenging and angiotensin converting enzyme inhibitory activities",
abstract = "The methanol-soluble sugar hydroxamic acid derivatives obtained during the preparation of alginic acid hydroxamates (AAH) were separated by BioSil-ODS HPLC column (10x250 mm) in acetonitrite: 0.05{\%} trifluoroacetic acid, 10:90 (V/V). The absorbance at 235 nm was set for monitoring β-elimination products. Each fraction was collected and assayed for hydroxamic acid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and inhibitory activity against angiotensin converting enzyme (ACE). The fraction with higher hydroxamic acid content was further separated by Sephadex G-15 column. Fractions containing both ACE inhibitory activity and DPPH scavenging activity overlapped with fractions containing high levels of hydroxamic acid derivatives. The chromatogram showed little tailing when judged by hydroxamic-acid content but significant tailing when judged by biological activity. Each fraction from above was further separated by silica-TLC in acetonitrite: distilled water, 95:5 (V/V). It was found that a methanol soluble fraction prepared from alginic acid hydroxamates (AAH) exhibited DPPH scavenging activity.",
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AB - The methanol-soluble sugar hydroxamic acid derivatives obtained during the preparation of alginic acid hydroxamates (AAH) were separated by BioSil-ODS HPLC column (10x250 mm) in acetonitrite: 0.05% trifluoroacetic acid, 10:90 (V/V). The absorbance at 235 nm was set for monitoring β-elimination products. Each fraction was collected and assayed for hydroxamic acid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and inhibitory activity against angiotensin converting enzyme (ACE). The fraction with higher hydroxamic acid content was further separated by Sephadex G-15 column. Fractions containing both ACE inhibitory activity and DPPH scavenging activity overlapped with fractions containing high levels of hydroxamic acid derivatives. The chromatogram showed little tailing when judged by hydroxamic-acid content but significant tailing when judged by biological activity. Each fraction from above was further separated by silica-TLC in acetonitrite: distilled water, 95:5 (V/V). It was found that a methanol soluble fraction prepared from alginic acid hydroxamates (AAH) exhibited DPPH scavenging activity.

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