Metabolomics of ginger essential oil against alcoholic fatty liver in mice

Chun Ting Liu, Rajasekaran Raghu, Shu Hsi Lin, San Yuan Wang, Ching Hua Kuo, Yufeng J. Tseng, Lee Yan Sheen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Fatty liver is significantly associated with hepatic cirrhosis and liver cancer. Excessive alcohol consumption causes alcoholic fatty liver disease (AFLD). Ginger has been reported to exhibit antioxidant potential and hepatoprotective activity. In the present study, a mouse model for AFLD was developed by employing male C57BL/6 mice that were fed an alcohol-containing liquid diet (Lieber-DeCarli diet) ad libitum. In the treatment groups, ginger essential oil (GEO) and citral were orally administered every day for 4 weeks. Serum biochemical analysis, antioxidant enzyme activity analysis, and histopathological evaluation revealed that GEO and citral exhibited hepatoprotective activity against AFLD. Metabolites in serum samples were profiled by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). Metabolomic data indicated the amounts of metabolites such as d-glucurono-6,3-lactone, glycerol-3-phosphate, pyruvic acid, lithocholic acid, 2-pyrocatechuic acid, and prostaglandin E1 were increased after alcohol administration, but the levels were recovered in treatment groups. The analysis indicated that ginger possesses hepatoprotective properties against AFLD. Furthermore, these metabolites can serve as early noninvasive candidate biomarkers in the clinical application of AFLD for health management.

Original languageEnglish
Pages (from-to)11231-11240
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number46
DOIs
Publication statusPublished - Nov 20 2013
Externally publishedYes

Fingerprint

Alcoholic Fatty Liver
Ginger
Alcoholic Liver Diseases
Metabolomics
ginger
fatty liver
metabolomics
Volatile Oils
citral
Liver
liver diseases
essential oils
hepatoprotective effect
mice
Metabolites
Liver Neoplasms
metabolites
Alcohols
Antioxidants
Nutrition

Keywords

  • AFLD
  • biomarker
  • ginger
  • Lieber-DeCarli diet
  • metabolomics

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Liu, C. T., Raghu, R., Lin, S. H., Wang, S. Y., Kuo, C. H., Tseng, Y. J., & Sheen, L. Y. (2013). Metabolomics of ginger essential oil against alcoholic fatty liver in mice. Journal of Agricultural and Food Chemistry, 61(46), 11231-11240. https://doi.org/10.1021/jf403523g

Metabolomics of ginger essential oil against alcoholic fatty liver in mice. / Liu, Chun Ting; Raghu, Rajasekaran; Lin, Shu Hsi; Wang, San Yuan; Kuo, Ching Hua; Tseng, Yufeng J.; Sheen, Lee Yan.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 46, 20.11.2013, p. 11231-11240.

Research output: Contribution to journalArticle

Liu, Chun Ting ; Raghu, Rajasekaran ; Lin, Shu Hsi ; Wang, San Yuan ; Kuo, Ching Hua ; Tseng, Yufeng J. ; Sheen, Lee Yan. / Metabolomics of ginger essential oil against alcoholic fatty liver in mice. In: Journal of Agricultural and Food Chemistry. 2013 ; Vol. 61, No. 46. pp. 11231-11240.
@article{f4f35c0d8cb54b20b523e2748f54d635,
title = "Metabolomics of ginger essential oil against alcoholic fatty liver in mice",
abstract = "Fatty liver is significantly associated with hepatic cirrhosis and liver cancer. Excessive alcohol consumption causes alcoholic fatty liver disease (AFLD). Ginger has been reported to exhibit antioxidant potential and hepatoprotective activity. In the present study, a mouse model for AFLD was developed by employing male C57BL/6 mice that were fed an alcohol-containing liquid diet (Lieber-DeCarli diet) ad libitum. In the treatment groups, ginger essential oil (GEO) and citral were orally administered every day for 4 weeks. Serum biochemical analysis, antioxidant enzyme activity analysis, and histopathological evaluation revealed that GEO and citral exhibited hepatoprotective activity against AFLD. Metabolites in serum samples were profiled by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). Metabolomic data indicated the amounts of metabolites such as d-glucurono-6,3-lactone, glycerol-3-phosphate, pyruvic acid, lithocholic acid, 2-pyrocatechuic acid, and prostaglandin E1 were increased after alcohol administration, but the levels were recovered in treatment groups. The analysis indicated that ginger possesses hepatoprotective properties against AFLD. Furthermore, these metabolites can serve as early noninvasive candidate biomarkers in the clinical application of AFLD for health management.",
keywords = "AFLD, biomarker, ginger, Lieber-DeCarli diet, metabolomics",
author = "Liu, {Chun Ting} and Rajasekaran Raghu and Lin, {Shu Hsi} and Wang, {San Yuan} and Kuo, {Ching Hua} and Tseng, {Yufeng J.} and Sheen, {Lee Yan}",
year = "2013",
month = "11",
day = "20",
doi = "10.1021/jf403523g",
language = "English",
volume = "61",
pages = "11231--11240",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "46",

}

TY - JOUR

T1 - Metabolomics of ginger essential oil against alcoholic fatty liver in mice

AU - Liu, Chun Ting

AU - Raghu, Rajasekaran

AU - Lin, Shu Hsi

AU - Wang, San Yuan

AU - Kuo, Ching Hua

AU - Tseng, Yufeng J.

AU - Sheen, Lee Yan

PY - 2013/11/20

Y1 - 2013/11/20

N2 - Fatty liver is significantly associated with hepatic cirrhosis and liver cancer. Excessive alcohol consumption causes alcoholic fatty liver disease (AFLD). Ginger has been reported to exhibit antioxidant potential and hepatoprotective activity. In the present study, a mouse model for AFLD was developed by employing male C57BL/6 mice that were fed an alcohol-containing liquid diet (Lieber-DeCarli diet) ad libitum. In the treatment groups, ginger essential oil (GEO) and citral were orally administered every day for 4 weeks. Serum biochemical analysis, antioxidant enzyme activity analysis, and histopathological evaluation revealed that GEO and citral exhibited hepatoprotective activity against AFLD. Metabolites in serum samples were profiled by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). Metabolomic data indicated the amounts of metabolites such as d-glucurono-6,3-lactone, glycerol-3-phosphate, pyruvic acid, lithocholic acid, 2-pyrocatechuic acid, and prostaglandin E1 were increased after alcohol administration, but the levels were recovered in treatment groups. The analysis indicated that ginger possesses hepatoprotective properties against AFLD. Furthermore, these metabolites can serve as early noninvasive candidate biomarkers in the clinical application of AFLD for health management.

AB - Fatty liver is significantly associated with hepatic cirrhosis and liver cancer. Excessive alcohol consumption causes alcoholic fatty liver disease (AFLD). Ginger has been reported to exhibit antioxidant potential and hepatoprotective activity. In the present study, a mouse model for AFLD was developed by employing male C57BL/6 mice that were fed an alcohol-containing liquid diet (Lieber-DeCarli diet) ad libitum. In the treatment groups, ginger essential oil (GEO) and citral were orally administered every day for 4 weeks. Serum biochemical analysis, antioxidant enzyme activity analysis, and histopathological evaluation revealed that GEO and citral exhibited hepatoprotective activity against AFLD. Metabolites in serum samples were profiled by high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). Metabolomic data indicated the amounts of metabolites such as d-glucurono-6,3-lactone, glycerol-3-phosphate, pyruvic acid, lithocholic acid, 2-pyrocatechuic acid, and prostaglandin E1 were increased after alcohol administration, but the levels were recovered in treatment groups. The analysis indicated that ginger possesses hepatoprotective properties against AFLD. Furthermore, these metabolites can serve as early noninvasive candidate biomarkers in the clinical application of AFLD for health management.

KW - AFLD

KW - biomarker

KW - ginger

KW - Lieber-DeCarli diet

KW - metabolomics

UR - http://www.scopus.com/inward/record.url?scp=84888330020&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888330020&partnerID=8YFLogxK

U2 - 10.1021/jf403523g

DO - 10.1021/jf403523g

M3 - Article

C2 - 24171385

AN - SCOPUS:84888330020

VL - 61

SP - 11231

EP - 11240

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 46

ER -