Metabolomics investigation of exercise-modulated changes in metabolism in rat liver after exhaustive and endurance exercises

Chi Chang Huang, Wan Teng Lin, Feng Lin Hsu, Pi Wen Tsai, Chia Chung Hou

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Exhaustive exercise and endurance exercise training modify the physiological status of the body differently. The present study aimed to evaluate the alteration in biochemical composition with exhaustive and endurance exercises in rats using metabolomics strategy. The metabolite profile of liver tissue was investigated on gas chromatography-mass spectrometry (GC-MS). Data further underwent partial least-squares-discriminant analysis (PLS-DA) to compare the effects on metabolites in sedentary control, exhaustively exercised and endurance trained rats. GC-MS detected 115 highly reproducible peaks in chromatograms from individual liver tissue extracts, and we identified 55 of them. The three groups showed significant differences in metabolic profile. Changes in liver metabolism involved metabolites such as amino acids, fatty acids, organic acids, and carbohydrates. Endurance training elevated the greater rate of tricarboxylic acid cycle and antioxidant activity, and exhaustive exercise led to accumulated urea markers and an inflammation response in liver. In addition, GC-MS-based metabolomic analysis is a promising tool to investigate a pathological status with different exercise programs.

Original languageEnglish
Pages (from-to)557-566
Number of pages10
JournalEuropean Journal of Applied Physiology
Volume108
Issue number3
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Metabolomics
Gas Chromatography-Mass Spectrometry
Liver
Liver Extracts
Tissue Extracts
Citric Acid Cycle
Metabolome
Discriminant Analysis
Least-Squares Analysis
Urea
Fatty Acids
Antioxidants
Carbohydrates
Exercise
Inflammation
Amino Acids
Acids

Keywords

  • Exercise training
  • Exhaustive exercise
  • Liver
  • Metabolomics
  • PLS-DA

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Orthopedics and Sports Medicine
  • Physiology (medical)

Cite this

Metabolomics investigation of exercise-modulated changes in metabolism in rat liver after exhaustive and endurance exercises. / Huang, Chi Chang; Lin, Wan Teng; Hsu, Feng Lin; Tsai, Pi Wen; Hou, Chia Chung.

In: European Journal of Applied Physiology, Vol. 108, No. 3, 02.2010, p. 557-566.

Research output: Contribution to journalArticle

Huang, Chi Chang ; Lin, Wan Teng ; Hsu, Feng Lin ; Tsai, Pi Wen ; Hou, Chia Chung. / Metabolomics investigation of exercise-modulated changes in metabolism in rat liver after exhaustive and endurance exercises. In: European Journal of Applied Physiology. 2010 ; Vol. 108, No. 3. pp. 557-566.
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