Metabolomic characterization of laborers exposed to welding fumes

Ching Hua Kuo, Kuo Ching Wang, Tze Feng Tian, Mong Hsun Tsai, Yin Mei Chiung, Chun Ming Hsiech, Sung Jeng Tsai, San Yuan Wang, Dong Ming Tsai, Chiang Ching Huang, Y. Jane Tseng

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The complex composition of welding fumes, multiplicity of molecular targets, diverse cellular effects, and lifestyles associated with laborers vastly complicate the assessment of welding fume exposure. The urinary metabolomic profiles of 35 male welders and 16 male office workers at a Taiwanese shipyard were characterized via 1H NMR spectroscopy and pattern recognition methods. Blood samples for the same 51 individuals were also collected, and the expression levels of the cytokines and other inflammatory markers were examined. This study dichotomized the welding exposure variable into high (welders) versus low (office workers) exposures to examine the differences of continuous outcome markers -metabolites and inflammatory markers - between the two groups. Fume particle assessments showed that welders were exposed to different concentrations of chromium, nickel, and manganese particles. Multivariate statistical analysis of urinary metabolomic patterns showed higher levels of glycine, taurine, betaine/TMAO, serine, S-sulfocysteine, hippurate, gluconate, creatinine, and acetone and lower levels of creatine among welders, while only TNF-α was significantly associated with welding fume exposure among all cytokines and other inflammatory markers measured. Of the identified metabolites, the higher levels of glycine, taurine, and betaine among welders were suspected to play some roles in modulating inflammatory and oxidative tissue injury processes. In this metabolomics experiment, we also discovered that the association of the identified metabolites with welding exposure was confounded by smoking, but not with drinking, which is a finding consistent with known modified response of inflammatory markers among smokers. Our results correspond with prior studies that utilized nonmetabolomic analytical techniques and suggest that the metabolomic profiling is an efficient method to characterize the overall effect of welding fume exposure and other confounders.

Original languageEnglish
Pages (from-to)676-686
Number of pages11
JournalChemical Research in Toxicology
Volume25
Issue number3
DOIs
Publication statusPublished - Mar 19 2012
Externally publishedYes

Fingerprint

Welding
Fumes
Metabolomics
Metabolites
Betaine
Taurine
Glycine
Cytokines
Shipyards
Creatine
Chromium
Manganese
Acetone
Nickel
Serine
Nuclear magnetic resonance spectroscopy
Drinking
Pattern recognition
Life Style
Creatinine

ASJC Scopus subject areas

  • Toxicology

Cite this

Kuo, C. H., Wang, K. C., Tian, T. F., Tsai, M. H., Chiung, Y. M., Hsiech, C. M., ... Tseng, Y. J. (2012). Metabolomic characterization of laborers exposed to welding fumes. Chemical Research in Toxicology, 25(3), 676-686. https://doi.org/10.1021/tx200465e

Metabolomic characterization of laborers exposed to welding fumes. / Kuo, Ching Hua; Wang, Kuo Ching; Tian, Tze Feng; Tsai, Mong Hsun; Chiung, Yin Mei; Hsiech, Chun Ming; Tsai, Sung Jeng; Wang, San Yuan; Tsai, Dong Ming; Huang, Chiang Ching; Tseng, Y. Jane.

In: Chemical Research in Toxicology, Vol. 25, No. 3, 19.03.2012, p. 676-686.

Research output: Contribution to journalArticle

Kuo, CH, Wang, KC, Tian, TF, Tsai, MH, Chiung, YM, Hsiech, CM, Tsai, SJ, Wang, SY, Tsai, DM, Huang, CC & Tseng, YJ 2012, 'Metabolomic characterization of laborers exposed to welding fumes', Chemical Research in Toxicology, vol. 25, no. 3, pp. 676-686. https://doi.org/10.1021/tx200465e
Kuo CH, Wang KC, Tian TF, Tsai MH, Chiung YM, Hsiech CM et al. Metabolomic characterization of laborers exposed to welding fumes. Chemical Research in Toxicology. 2012 Mar 19;25(3):676-686. https://doi.org/10.1021/tx200465e
Kuo, Ching Hua ; Wang, Kuo Ching ; Tian, Tze Feng ; Tsai, Mong Hsun ; Chiung, Yin Mei ; Hsiech, Chun Ming ; Tsai, Sung Jeng ; Wang, San Yuan ; Tsai, Dong Ming ; Huang, Chiang Ching ; Tseng, Y. Jane. / Metabolomic characterization of laborers exposed to welding fumes. In: Chemical Research in Toxicology. 2012 ; Vol. 25, No. 3. pp. 676-686.
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