Metabolomic assessment of arsenite toxicity and novel biomarker discovery in early development of zebrafish embryos

Szu Yuan Wu, Nam Nhut Phan, Shih Hsin Ho, Yu Heng Lai, Chih Hung Tsai, Chung Han Yang, Han Gang Yu, Jung Chieh Wang, Pung Ling Huang, Yen Chang Lin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Context: Arsenic poisoning commonly occurs through exposure to water contaminated with arsenic and causes long-term symptoms. Of all the arsenic derivatives, arsenite is the one of the most toxic compounds. However, the toxicity of arsenite during developmental stages is still unclear. Objective: In this study, we performed a metabolomic analysis of arsenite responses in embryonic zebrafish. Materials and methods: Embryonic zebrafish were used as an animal model in this study. They were exposed to sodium arsenite under different concentrations (0.5, 1.0, 2.0, and 5.0 mg/L) in 24 h, 48 h and 72 h post fertilization. Changes in morphology were observed through a light microscope. Changes in metabolomics were identified using an ultraperformance liquid chromatography quadrupole time-of-flight system. Results: The IC50 range was 0.75 ± 0.25 mg/L. Compared with the control group, the embryonic lethality rate decreased to 33.3% under 1.0 mg/L of arsenite treatment, whereas it decreased to 20.0% under 2.0 mg/L of arsenite treatment. Numerous body axis curvatures were also observed under treatment with 2.0 and 5.0 mg/L of arsenic. Pericardium and yolk sac edema were randomly discovered and found to worsen over time. Moreover, the 10 metabolites with the highest variable importance in projection score were identified as potential biomarkers for arsenic exposure. Conclusion: Arsenic exposure not only leads to a change in the morphology of embryonic zebrafish but also disturbs the metabolism of zebrafish in early developmental stages.

Original languageEnglish
Pages (from-to)116-122
Number of pages7
JournalToxicology Letters
Volume290
DOIs
Publication statusPublished - Jun 15 2018

Fingerprint

Metabolomics
Arsenic
Biomarkers
Zebrafish
Embryonic Development
Toxicity
Arsenic Poisoning
Yolk Sac
Poisons
Pericardium
Fertilization
Liquid Chromatography
Liquid chromatography
Inhibitory Concentration 50
Metabolites
Edema
Metabolism
Animal Models
arsenite
Animals

Keywords

  • Embryonic zebrafish
  • Metabolomic analysis
  • Sodium arsenite

ASJC Scopus subject areas

  • Toxicology

Cite this

Metabolomic assessment of arsenite toxicity and novel biomarker discovery in early development of zebrafish embryos. / Wu, Szu Yuan; Phan, Nam Nhut; Ho, Shih Hsin; Lai, Yu Heng; Tsai, Chih Hung; Yang, Chung Han; Yu, Han Gang; Wang, Jung Chieh; Huang, Pung Ling; Lin, Yen Chang.

In: Toxicology Letters, Vol. 290, 15.06.2018, p. 116-122.

Research output: Contribution to journalArticle

Wu, SY, Phan, NN, Ho, SH, Lai, YH, Tsai, CH, Yang, CH, Yu, HG, Wang, JC, Huang, PL & Lin, YC 2018, 'Metabolomic assessment of arsenite toxicity and novel biomarker discovery in early development of zebrafish embryos', Toxicology Letters, vol. 290, pp. 116-122. https://doi.org/10.1016/j.toxlet.2018.03.014
Wu, Szu Yuan ; Phan, Nam Nhut ; Ho, Shih Hsin ; Lai, Yu Heng ; Tsai, Chih Hung ; Yang, Chung Han ; Yu, Han Gang ; Wang, Jung Chieh ; Huang, Pung Ling ; Lin, Yen Chang. / Metabolomic assessment of arsenite toxicity and novel biomarker discovery in early development of zebrafish embryos. In: Toxicology Letters. 2018 ; Vol. 290. pp. 116-122.
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abstract = "Context: Arsenic poisoning commonly occurs through exposure to water contaminated with arsenic and causes long-term symptoms. Of all the arsenic derivatives, arsenite is the one of the most toxic compounds. However, the toxicity of arsenite during developmental stages is still unclear. Objective: In this study, we performed a metabolomic analysis of arsenite responses in embryonic zebrafish. Materials and methods: Embryonic zebrafish were used as an animal model in this study. They were exposed to sodium arsenite under different concentrations (0.5, 1.0, 2.0, and 5.0 mg/L) in 24 h, 48 h and 72 h post fertilization. Changes in morphology were observed through a light microscope. Changes in metabolomics were identified using an ultraperformance liquid chromatography quadrupole time-of-flight system. Results: The IC50 range was 0.75 ± 0.25 mg/L. Compared with the control group, the embryonic lethality rate decreased to 33.3{\%} under 1.0 mg/L of arsenite treatment, whereas it decreased to 20.0{\%} under 2.0 mg/L of arsenite treatment. Numerous body axis curvatures were also observed under treatment with 2.0 and 5.0 mg/L of arsenic. Pericardium and yolk sac edema were randomly discovered and found to worsen over time. Moreover, the 10 metabolites with the highest variable importance in projection score were identified as potential biomarkers for arsenic exposure. Conclusion: Arsenic exposure not only leads to a change in the morphology of embryonic zebrafish but also disturbs the metabolism of zebrafish in early developmental stages.",
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AU - Wu, Szu Yuan

AU - Phan, Nam Nhut

AU - Ho, Shih Hsin

AU - Lai, Yu Heng

AU - Tsai, Chih Hung

AU - Yang, Chung Han

AU - Yu, Han Gang

AU - Wang, Jung Chieh

AU - Huang, Pung Ling

AU - Lin, Yen Chang

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N2 - Context: Arsenic poisoning commonly occurs through exposure to water contaminated with arsenic and causes long-term symptoms. Of all the arsenic derivatives, arsenite is the one of the most toxic compounds. However, the toxicity of arsenite during developmental stages is still unclear. Objective: In this study, we performed a metabolomic analysis of arsenite responses in embryonic zebrafish. Materials and methods: Embryonic zebrafish were used as an animal model in this study. They were exposed to sodium arsenite under different concentrations (0.5, 1.0, 2.0, and 5.0 mg/L) in 24 h, 48 h and 72 h post fertilization. Changes in morphology were observed through a light microscope. Changes in metabolomics were identified using an ultraperformance liquid chromatography quadrupole time-of-flight system. Results: The IC50 range was 0.75 ± 0.25 mg/L. Compared with the control group, the embryonic lethality rate decreased to 33.3% under 1.0 mg/L of arsenite treatment, whereas it decreased to 20.0% under 2.0 mg/L of arsenite treatment. Numerous body axis curvatures were also observed under treatment with 2.0 and 5.0 mg/L of arsenic. Pericardium and yolk sac edema were randomly discovered and found to worsen over time. Moreover, the 10 metabolites with the highest variable importance in projection score were identified as potential biomarkers for arsenic exposure. Conclusion: Arsenic exposure not only leads to a change in the morphology of embryonic zebrafish but also disturbs the metabolism of zebrafish in early developmental stages.

AB - Context: Arsenic poisoning commonly occurs through exposure to water contaminated with arsenic and causes long-term symptoms. Of all the arsenic derivatives, arsenite is the one of the most toxic compounds. However, the toxicity of arsenite during developmental stages is still unclear. Objective: In this study, we performed a metabolomic analysis of arsenite responses in embryonic zebrafish. Materials and methods: Embryonic zebrafish were used as an animal model in this study. They were exposed to sodium arsenite under different concentrations (0.5, 1.0, 2.0, and 5.0 mg/L) in 24 h, 48 h and 72 h post fertilization. Changes in morphology were observed through a light microscope. Changes in metabolomics were identified using an ultraperformance liquid chromatography quadrupole time-of-flight system. Results: The IC50 range was 0.75 ± 0.25 mg/L. Compared with the control group, the embryonic lethality rate decreased to 33.3% under 1.0 mg/L of arsenite treatment, whereas it decreased to 20.0% under 2.0 mg/L of arsenite treatment. Numerous body axis curvatures were also observed under treatment with 2.0 and 5.0 mg/L of arsenic. Pericardium and yolk sac edema were randomly discovered and found to worsen over time. Moreover, the 10 metabolites with the highest variable importance in projection score were identified as potential biomarkers for arsenic exposure. Conclusion: Arsenic exposure not only leads to a change in the morphology of embryonic zebrafish but also disturbs the metabolism of zebrafish in early developmental stages.

KW - Embryonic zebrafish

KW - Metabolomic analysis

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