Metabolomic analysis of the effects of motorcycle exhaust on rat testes and liver

Ching Yu Lin, Shang Ying Wu, Hao Jan Liang, Yi Chun Liu, Tzuu Huei Ueng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Motorcycles are one of the primary means of transportation in many Asian metropolitan areas. Exposure to motorcycle exhaust (ME) has been shown to cause male reproductive toxicity in a rat inhalation model. In the present study, the molecular mechanisms underlying ME-induced male reproductive toxicity were examined in a rat model using nuclear magnetic resonance (NMR)-based metabolomics. Rats were treated with 1:10-diluted ME for 1 hour in the morning and 1 hour in the afternoon, daily, via head-nose-only exposure chambers, for a period of four weeks. Half of the ME-exposed rats were co-treated with vitamin E to examine its protective effects. Both hydrophilic and hydrophobic metabolites from the testes and liver were extracted for 1H and J-resolved NMR analyses. The obtained NMR spectra were converted into series of spectral binned areas and subjected to orthogonal projections to latent structure-discriminant analyses. Decreased spermatid counts and elevated levels of betaine in the testes of rats in the ME-treated group were partially attenuated by vitamin E co-treatment. Although it was not statistically significant, we observed a consistent trend of partial reversal of the elevated levels of alanine, glycine, leucine, tyrosine, and valine in the testes of rats in the ME-treated group due to vitamin E co-treatment. Decreases in testicular ethanolamine and inositol were only significant in the group co-treated with ME and vitamin E. The testicular toxicity of ME may be due to alterations in lipid- and energy-related metabolism. We conclude that ME generates greater metabolic responses in the testes than in the liver. Treatment with vitamin E can partially reverse the molecular events in the testes.

Original languageEnglish
Pages (from-to)1714-1725
Number of pages12
JournalAerosol and Air Quality Research
Volume14
Issue number6
DOIs
Publication statusPublished - Oct 1 2014

Fingerprint

Motorcycles
Liver
Rats
Vitamins
vitamin
Vitamin E
Toxicity
nuclear magnetic resonance
Nuclear magnetic resonance
toxicity
Metabolomics
analysis
effect
motorcycle
Betaines
Ethanolamines
Betaine
Ethanolamine
Valine
Inositol

Keywords

  • Male reproductive toxicity
  • Metabolomics
  • Motorcycle exhaust
  • Nuclear magnetic resonance
  • Testes

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

Cite this

Metabolomic analysis of the effects of motorcycle exhaust on rat testes and liver. / Lin, Ching Yu; Wu, Shang Ying; Liang, Hao Jan; Liu, Yi Chun; Ueng, Tzuu Huei.

In: Aerosol and Air Quality Research, Vol. 14, No. 6, 01.10.2014, p. 1714-1725.

Research output: Contribution to journalArticle

Lin, Ching Yu ; Wu, Shang Ying ; Liang, Hao Jan ; Liu, Yi Chun ; Ueng, Tzuu Huei. / Metabolomic analysis of the effects of motorcycle exhaust on rat testes and liver. In: Aerosol and Air Quality Research. 2014 ; Vol. 14, No. 6. pp. 1714-1725.
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