Metabolomic analysis of complex Chinese remedies

Examples of induced nephrotoxicity in the mouse from a series of remedies containing aristolochic acid

Dong Ming Tsai, Jaw Jou Kang, Shoei Sheng Lee, San Yuan Wang, I. Lin Tsai, Guan Yuan Chen, Hsiao Wei Liao, Li Wei-Chu, Ching Hua Kuo, Y. Jane Tseng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aristolochic acid nephropathy is caused by aristolochic acid (AA) and AA-containing herbs. In traditional Chinese medicine, a principle called "Jun-Chen-Zou-Shi" may be utilized to construct a remedial herbal formula that attempts to mitigate the toxicity of the main ingredient. This study used Bu-Fei-A-Jiao-Tang (BFAJT) to test if the compound remedy based on a principle of "Jun-Chen-Zou-Shi" can decrease the toxicity of AA-containing herbs. We compared the three toxicities of AA standard, Madouling (an Aristolochia herb), and a herbal formula BFAJT. AA standard was given for BALB/c mice at a dose of 5 mg/kg bw/day or 7.5 mg/kg bw/day for 10 days. Madouling and BFAJT were given at an equivalence of AA 0.5 mg/kg bw/day for 21 days. Nephrotoxicity was evaluated by metabolomics and histopathology. The urinary metabolomics profiles were characterized by 1H NMR spectroscopy. The spectral data was analyzed with partial least squares discriminant analysis, and the significant differential metabolites between groups were identified. The result showed different degrees of acute renal tubular injuries, and metabolomics analysis found that the kidney injuries were focused in proximal renal tubules. Both metabolomics and pathological studies revealed that AA standard, Madouling, and BFAJT were all nephrotoxicants. The compositions of the compound remedy did not diminish the nephrotoxicity caused by AA.

Original languageEnglish
Article number263757
JournalEvidence-based Complementary and Alternative Medicine
Volume2013
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Metabolomics
Aristolochia
aristolochic acid I
Proximal Kidney Tubule
Chinese Traditional Medicine
Discriminant Analysis
Least-Squares Analysis
Acute Kidney Injury
Magnetic Resonance Spectroscopy
Kidney
Wounds and Injuries

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Metabolomic analysis of complex Chinese remedies : Examples of induced nephrotoxicity in the mouse from a series of remedies containing aristolochic acid. / Tsai, Dong Ming; Kang, Jaw Jou; Lee, Shoei Sheng; Wang, San Yuan; Tsai, I. Lin; Chen, Guan Yuan; Liao, Hsiao Wei; Wei-Chu, Li; Kuo, Ching Hua; Tseng, Y. Jane.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2013, 263757, 2013.

Research output: Contribution to journalArticle

Tsai, Dong Ming ; Kang, Jaw Jou ; Lee, Shoei Sheng ; Wang, San Yuan ; Tsai, I. Lin ; Chen, Guan Yuan ; Liao, Hsiao Wei ; Wei-Chu, Li ; Kuo, Ching Hua ; Tseng, Y. Jane. / Metabolomic analysis of complex Chinese remedies : Examples of induced nephrotoxicity in the mouse from a series of remedies containing aristolochic acid. In: Evidence-based Complementary and Alternative Medicine. 2013 ; Vol. 2013.
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