Abstract
The effects of the metabolites of Scutellariae Radix (SR), a traditional anti-inflammatory oriental herbal medicine, on hypoxia/reoxygenation (H/R)-induced inflammation and apoptosis of human umbilical vein endothelial cells (HUVECs) were investigated. SR metabolites (SRMs) were prepared from the sulfatase/β-glucuronidase-added serum of SR-decoction-fed rats and examined using highperformance liquid chromatography. HUVECs were put into a mechanical device conditioned with hypoxia (94 %N2, 5 % CO2, and 1 %O2) for 4 h (H4), and then reoxygenation (room air with 5 % CO2) for 4 h (R4), alone or in the presence of SRMs or vitamin C. Cell adhesion and apoptosis were detected using the enzyme-linked immunosorbent assay. Intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), caspase-3, and heme oxygenase-1 (HO-1) were evaluated using western blotting. COX-2 small hairpin RNA (shRNA) and HO-1 shRNA were used to evaluate the relationship of COX-2 and HO-1 to cell apoptosis. THP-1 adhesion, and ICAM-1 and COX-2 expression increased at H4 and H4/R4, but decreased at H8, and could be ameliorated by SRMs in a dose-dependent manner. Notedly, SRMs effectively attenuated COX-2-mediated cell apoptosis, which was enhanced byHO-1 shRNA, especially atH4/R4 injury. In conclusion, in a hypoxia chamber that mimics the hypoxic condition in acute coronary syndrome, SRMs protected H/Rinduced inflammation and the death of endothelial cells via oxidative stress reduction.
| Original language | English |
|---|---|
| Pages (from-to) | 492-499 |
| Number of pages | 8 |
| Journal | Journal of Medical and Biological Engineering |
| Volume | 35 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Apoptosis
- Cyclooxygenase-2
- Endothelial cells
- Hypoxia
- Inflammation
- Scutellariae radix
ASJC Scopus subject areas
- Biomedical Engineering
- Medicine(all)
Cite this
Metabolites of scutellariae radix inhibit injury of endothelial cells in hypoxia device. / Chao, Chia Lun; Lin, Shiuan Pey; Hou, Yu Chi; Chao, Pei Dawn Lee; Chang, Nen Chung; Huang, Yu Ching; Ho, Feng Ming.
In: Journal of Medical and Biological Engineering, Vol. 35, No. 4, 2015, p. 492-499.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metabolites of scutellariae radix inhibit injury of endothelial cells in hypoxia device
AU - Chao, Chia Lun
AU - Lin, Shiuan Pey
AU - Hou, Yu Chi
AU - Chao, Pei Dawn Lee
AU - Chang, Nen Chung
AU - Huang, Yu Ching
AU - Ho, Feng Ming
PY - 2015
Y1 - 2015
N2 - The effects of the metabolites of Scutellariae Radix (SR), a traditional anti-inflammatory oriental herbal medicine, on hypoxia/reoxygenation (H/R)-induced inflammation and apoptosis of human umbilical vein endothelial cells (HUVECs) were investigated. SR metabolites (SRMs) were prepared from the sulfatase/β-glucuronidase-added serum of SR-decoction-fed rats and examined using highperformance liquid chromatography. HUVECs were put into a mechanical device conditioned with hypoxia (94 %N2, 5 % CO2, and 1 %O2) for 4 h (H4), and then reoxygenation (room air with 5 % CO2) for 4 h (R4), alone or in the presence of SRMs or vitamin C. Cell adhesion and apoptosis were detected using the enzyme-linked immunosorbent assay. Intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), caspase-3, and heme oxygenase-1 (HO-1) were evaluated using western blotting. COX-2 small hairpin RNA (shRNA) and HO-1 shRNA were used to evaluate the relationship of COX-2 and HO-1 to cell apoptosis. THP-1 adhesion, and ICAM-1 and COX-2 expression increased at H4 and H4/R4, but decreased at H8, and could be ameliorated by SRMs in a dose-dependent manner. Notedly, SRMs effectively attenuated COX-2-mediated cell apoptosis, which was enhanced byHO-1 shRNA, especially atH4/R4 injury. In conclusion, in a hypoxia chamber that mimics the hypoxic condition in acute coronary syndrome, SRMs protected H/Rinduced inflammation and the death of endothelial cells via oxidative stress reduction.
AB - The effects of the metabolites of Scutellariae Radix (SR), a traditional anti-inflammatory oriental herbal medicine, on hypoxia/reoxygenation (H/R)-induced inflammation and apoptosis of human umbilical vein endothelial cells (HUVECs) were investigated. SR metabolites (SRMs) were prepared from the sulfatase/β-glucuronidase-added serum of SR-decoction-fed rats and examined using highperformance liquid chromatography. HUVECs were put into a mechanical device conditioned with hypoxia (94 %N2, 5 % CO2, and 1 %O2) for 4 h (H4), and then reoxygenation (room air with 5 % CO2) for 4 h (R4), alone or in the presence of SRMs or vitamin C. Cell adhesion and apoptosis were detected using the enzyme-linked immunosorbent assay. Intercellular adhesion molecule-1 (ICAM-1), cyclooxygenase-2 (COX-2), caspase-3, and heme oxygenase-1 (HO-1) were evaluated using western blotting. COX-2 small hairpin RNA (shRNA) and HO-1 shRNA were used to evaluate the relationship of COX-2 and HO-1 to cell apoptosis. THP-1 adhesion, and ICAM-1 and COX-2 expression increased at H4 and H4/R4, but decreased at H8, and could be ameliorated by SRMs in a dose-dependent manner. Notedly, SRMs effectively attenuated COX-2-mediated cell apoptosis, which was enhanced byHO-1 shRNA, especially atH4/R4 injury. In conclusion, in a hypoxia chamber that mimics the hypoxic condition in acute coronary syndrome, SRMs protected H/Rinduced inflammation and the death of endothelial cells via oxidative stress reduction.
KW - Apoptosis
KW - Cyclooxygenase-2
KW - Endothelial cells
KW - Hypoxia
KW - Inflammation
KW - Scutellariae radix
UR - http://www.scopus.com/inward/record.url?scp=84940388909&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84940388909&partnerID=8YFLogxK
U2 - 10.1007/s40846-015-0057-0
DO - 10.1007/s40846-015-0057-0
M3 - Article
AN - SCOPUS:84940388909
VL - 35
SP - 492
EP - 499
JO - Journal of Medical and Biological Engineering
JF - Journal of Medical and Biological Engineering
SN - 1609-0985
IS - 4
ER -