Metabolic Changes Associated With Cardiomyocyte Dedifferentiation Enable Adult Mammalian Cardiac Regeneration

Yuan-Yuan Cheng, Zachery Gregorich, Ray P Prajnamitra, David J Lundy, Ting-Yun Ma, Yu-Hsuan Huang, Yi-Chan Lee, Shu-Chian Rua, Jen-Hao Lin, Po-Ju Lin, Chiung Wen Kuo, Peilin Chen, Yu-Ting Yan, Rong Tian, Timothy J Kamp, Patrick C H Hsieh

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND: Cardiac regeneration after injury is limited by the low proliferative capacity of adult mammalian cardiomyocytes (CMs). However, certain animals readily regenerate lost myocardium through a process involving dedifferentiation, which unlocks their proliferative capacities.

METHODS: We bred mice with inducible, CM-specific expression of the Yamanaka factors, enabling adult CM reprogramming and dedifferentiation in vivo.

RESULTS: Two days after induction, adult CMs presented a dedifferentiated phenotype and increased proliferation in vivo. Microarray analysis revealed that upregulation of ketogenesis was central to this process. Adenovirus-driven HMGCS2 overexpression induced ketogenesis in adult CMs and recapitulated CM dedifferentiation and proliferation observed during partial reprogramming. This same phenomenon was found to occur after myocardial infarction, specifically in the border zone tissue, and HMGCS2 knockout mice showed impaired cardiac function and response to injury. Finally, we showed that exogenous HMGCS2 rescues cardiac function after ischemic injury.

CONCLUSIONS: Our data demonstrate the importance of HMGCS2-induced ketogenesis as a means to regulate metabolic response to CM injury, thus allowing cell dedifferentiation and proliferation as a regenerative response.

Original languageEnglish
Pages (from-to)1950-1967
Number of pages18
JournalCirculation
Volume146
Issue number25
Early online dateNov 24 2022
DOIs
Publication statusPublished - Dec 20 2022

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