Meta-analysis of public microarray datasets reveals voltage-gated calcium gene signatures in clinical cancer patients

Chih Yang Wang, Ming Derg Lai, Nam Nhut Phan, Zhengda Sun, Yen Chang Lin

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Voltage-gated calcium channels (VGCCs) are well documented to play roles in cell proliferation, migration, and apoptosis; however, whether VGCCs regulate the onset and progression of cancer is still under investigation. The VGCC family consists of five members, which are L-type, N-type, T-type, R-type and P/Q type. To date, no holistic approach has been used to screen VGCC family genes in different types of cancer. We analyzed the transcript expression of VGCCs in clinical cancer tissue samples by accessing ONCOMINE (www.oncomine.org), a web-based microarray database, to perform a systematic analysis. Every member of the VGCCs was examined across 21 different types of cancer by comparing mRNA expression in cancer to that in normal tissue. A previous study showed that altered expression of mRNA in cancer tissue may play an oncogenic role and promote tumor development; therefore, in the present findings, we focus only on the overexpression of VGCCs in different types of cancer. This bioinformatics analysis revealed that different subtypes of VGCCs (CACNA1C, CACNA1D, CACNA1B, CACNA1G, and CACNA1I) are implicated in the development and progression of diverse types of cancer and show dramatic up-regulation in breast cancer. CACNA1F only showed high expression in testis cancer, whereas CACNA1A, CACNA1C, and CACNA1D were highly expressed in most types of cancer. The current analysis revealed that specific VGCCs likely play essential roles in specific types of cancer. Collectively, we identified several VGCC targets and classified them according to different cancer subtypes for prospective studies on the underlying carcinogenic mechanisms. The present findings suggest that VGCCs are possible targets for prospective investigation in cancer treatment.

Original languageEnglish
Article numbere0125766
JournalPLoS ONE
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 6 2015
Externally publishedYes

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Calcium Channels
Microarrays
meta-analysis
calcium channels
Meta-Analysis
Genes
Calcium
calcium
neoplasms
Electric potential
Neoplasms
genes
Tissue
Datasets
Messenger RNA
Oncology
Cell proliferation
Bioinformatics
Testicular Neoplasms
Tumors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Meta-analysis of public microarray datasets reveals voltage-gated calcium gene signatures in clinical cancer patients. / Wang, Chih Yang; Lai, Ming Derg; Phan, Nam Nhut; Sun, Zhengda; Lin, Yen Chang.

In: PLoS ONE, Vol. 10, No. 7, e0125766, 06.07.2015.

Research output: Contribution to journalArticle

Wang, Chih Yang ; Lai, Ming Derg ; Phan, Nam Nhut ; Sun, Zhengda ; Lin, Yen Chang. / Meta-analysis of public microarray datasets reveals voltage-gated calcium gene signatures in clinical cancer patients. In: PLoS ONE. 2015 ; Vol. 10, No. 7.
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