Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells

Hon Man Liu, Si Han Wu, Chen Wen Lu, Ming Yao, Jong Kai Hsiao, Yann Hung, Yu Shen Lin, Chung Yuan Mou, Chung Shi Yang, Dong Ming Huang, Yao Chang Chen

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPICK&SiO 2) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO2-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO2.

Original languageEnglish
Pages (from-to)619-626
Number of pages8
JournalSmall
Volume4
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

Fingerprint

Magnetic resonance
Stem cells
Silicon Dioxide
Nanoparticles
Labeling
Coloring Agents
Stem Cells
Dyes
Silica
Magnetic Resonance Imaging
Fluorescein
Mesenchymal Stromal Cells
Imaging techniques
Cells
Osteocytes
Multicarrier modulation
Endocytosis
Iron oxides
Adipocytes
Imaging systems

Keywords

  • Biocompatibte materials
  • Magnetic resonance imaging
  • Mesoporous materials
  • Nanoparticles
  • Stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

Cite this

Liu, H. M., Wu, S. H., Lu, C. W., Yao, M., Hsiao, J. K., Hung, Y., ... Chen, Y. C. (2008). Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells. Small, 4(5), 619-626. https://doi.org/10.1002/smll.200700493

Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells. / Liu, Hon Man; Wu, Si Han; Lu, Chen Wen; Yao, Ming; Hsiao, Jong Kai; Hung, Yann; Lin, Yu Shen; Mou, Chung Yuan; Yang, Chung Shi; Huang, Dong Ming; Chen, Yao Chang.

In: Small, Vol. 4, No. 5, 05.2008, p. 619-626.

Research output: Contribution to journalArticle

Liu, HM, Wu, SH, Lu, CW, Yao, M, Hsiao, JK, Hung, Y, Lin, YS, Mou, CY, Yang, CS, Huang, DM & Chen, YC 2008, 'Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells', Small, vol. 4, no. 5, pp. 619-626. https://doi.org/10.1002/smll.200700493
Liu, Hon Man ; Wu, Si Han ; Lu, Chen Wen ; Yao, Ming ; Hsiao, Jong Kai ; Hung, Yann ; Lin, Yu Shen ; Mou, Chung Yuan ; Yang, Chung Shi ; Huang, Dong Ming ; Chen, Yao Chang. / Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells. In: Small. 2008 ; Vol. 4, No. 5. pp. 619-626.
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