Melatonin suppresses TPA-induced metastasis by downregulating matrix metalloproteinase-9 expression through JNK/SP-1 signaling in nasopharyngeal carcinoma

Hsin Yu Ho, Chiao Wen Lin, Ming Hsien Chien, Russel J. Reiter, Shih Chi Su, Yi Hsien Hsieh, Shun Fa Yang

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Nasopharyngeal carcinoma (NPC), a disease common in the South-East Asian population, has high lymph node metastatic ability. Melatonin, an endogenously produced substance present in animals, plants, fungi, and bacteria, has oncostatic activity via several mechanisms. The molecular mechanisms involved in melatonin-mediated tumor inhibitory potential are not completely defined. Here, we show that melatonin treatment inhibits TPA-induced cell motility by regulating the matrix metalloproteinase-9 (MMP-9) expression in NPC. We also identified the signaling cascade through which melatonin inhibits MMP-9 expression; this involves melatonin regulating the binding activity of the transcription factor specificity protein-1 (SP-1)-DNA. Our mechanistic analysis further reveals that the c-Jun N-terminal kinase/mitogen-activated protein kinase pathway is involved in the melatonin-mediated tumor suppressor activity. Furthermore, the findings indicate a functional link between melatonin-mediated MMP-9 regulation and tumor suppressing ability and provide new insights into the role of melatonin-induced molecular and epigenetic regulation of tumor growth. Thus, we conclude that melatonin suppresses the motility of NPC by regulating TPA-induced MMP-9 gene expression via inhibiting SP-1-DNA binding ability. The results provide a functional link between melatonin-mediated SP-1 regulation and the antimetastatic actions of melatonin on nasopharyngeal carcinoma.

Original languageEnglish
Pages (from-to)479-492
Number of pages14
JournalJournal of Pineal Research
Volume61
Issue number4
DOIs
Publication statusPublished - Nov 1 2016

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Matrix Metalloproteinase 9
Melatonin
Down-Regulation
Neoplasm Metastasis
Proteins
Neoplasms
Nasopharyngeal carcinoma
Sp1 Transcription Factor
JNK Mitogen-Activated Protein Kinases
DNA-Binding Proteins
Mitogen-Activated Protein Kinases
Epigenomics
Cell Movement
Fungi
Lymph Nodes
Bacteria
Gene Expression

Keywords

  • melatonin
  • metastasis
  • MMP
  • nasopharyngeal carcinoma
  • SP-1

ASJC Scopus subject areas

  • Endocrinology

Cite this

Melatonin suppresses TPA-induced metastasis by downregulating matrix metalloproteinase-9 expression through JNK/SP-1 signaling in nasopharyngeal carcinoma. / Ho, Hsin Yu; Lin, Chiao Wen; Chien, Ming Hsien; Reiter, Russel J.; Su, Shih Chi; Hsieh, Yi Hsien; Yang, Shun Fa.

In: Journal of Pineal Research, Vol. 61, No. 4, 01.11.2016, p. 479-492.

Research output: Contribution to journalArticle

Ho, Hsin Yu ; Lin, Chiao Wen ; Chien, Ming Hsien ; Reiter, Russel J. ; Su, Shih Chi ; Hsieh, Yi Hsien ; Yang, Shun Fa. / Melatonin suppresses TPA-induced metastasis by downregulating matrix metalloproteinase-9 expression through JNK/SP-1 signaling in nasopharyngeal carcinoma. In: Journal of Pineal Research. 2016 ; Vol. 61, No. 4. pp. 479-492.
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