Melatonin attenuates neuronal NADPH-d/NOS expression in the hypoglossal nucleus of adult rats following peripheral nerve injury

Hung Ming Chang, Eng A. Ling, June Horng Lue, Chen Yuan Wen, Jeng Yung Shieh

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Oxidative stress and massive production of nitric oxide (NO) have been implicated in the neuropathogenesis following peripheral nerve injury. This study was aimed to ascertain whether melatonin would exert its neuroprotective effect on the lesioned hypoglossal neurons after peripheral axotomy, since it is known to reduce the oxidative damage in a variety of experimental neuropathologies in which NO is involved. Right-sided hypoglossal nerve transection was performed in adult rats following which the animals were given two different doses of melatonin administered intraperitoneally for 3, 7, 14, 21 and 30 successive days. Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and neuronal nitric oxide synthase (nNOS) immunohistochemistry were carried out to detect the neuronal NADPH-d/NOS expression in the hypoglossal nucleus (HN). At various time intervals following axotomy, the neurons in the affected HN were induced to express NADPH-d/NOS reactivity on the lesioned side peaking at 14 days. However, the enzyme expression was markedly depressed by melatonin treatment in a dose-dependent manner in terms of frequency of labelled neurons and staining intensity. It is suggested that the suppressive effect of melatonin on NADPH-d/NOS expression may be attributed to its antioxidant properties. Hence, in consideration of therapeutic strategies for reducing the oxidative stress following peripheral nerve injury, melatonin may prove to be beneficial. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)243-251
Number of pages9
JournalBrain Research
Volume873
Issue number2
DOIs
Publication statusPublished - Aug 11 2000
Externally publishedYes

Fingerprint

Peripheral Nerve Injuries
Melatonin
NADP
Axotomy
Neurons
Hypoglossal Nerve Injuries
Nitric Oxide
Oxidative Stress
Nitric Oxide Synthase Type I
Neuroprotective Agents
Antioxidants
Immunohistochemistry
Staining and Labeling
Enzymes
Therapeutics

Keywords

  • Histochemistry
  • Hypoglossal nucleus
  • Immunohistochemistry
  • Melatonin
  • Nitric oxide
  • Peripheral nerve injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Melatonin attenuates neuronal NADPH-d/NOS expression in the hypoglossal nucleus of adult rats following peripheral nerve injury. / Chang, Hung Ming; Ling, Eng A.; Lue, June Horng; Wen, Chen Yuan; Shieh, Jeng Yung.

In: Brain Research, Vol. 873, No. 2, 11.08.2000, p. 243-251.

Research output: Contribution to journalArticle

Chang, Hung Ming ; Ling, Eng A. ; Lue, June Horng ; Wen, Chen Yuan ; Shieh, Jeng Yung. / Melatonin attenuates neuronal NADPH-d/NOS expression in the hypoglossal nucleus of adult rats following peripheral nerve injury. In: Brain Research. 2000 ; Vol. 873, No. 2. pp. 243-251.
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