MeDIP-on-chip for methylation profiling

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlations with biological events or disease progression. Methylated DNA immunoprecipitation (MeDIP) uses an antibody that efficiently enriches methylated DNA fragments for downstream locus-specific or genome-wide analyses. MeDIP-on-Chip uses the MeDIP approach in combination with a tiling array for the investigation of genome-wide DNA methylation patterns (or DNA methylomics). The following protocol describes the application of MeDIP to the hybridization of DNA microarrays and data analysis.

Original languageEnglish
Title of host publicationCervical Cancer: Methods and Protocols
PublisherSpringer New York
Pages281-290
Number of pages10
ISBN (Print)9781493920136, 9781493920129
DOIs
Publication statusPublished - Oct 27 2014

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Keywords

  • 5-methylcytidine
  • 5-methylcytidine antibody
  • CpG islands
  • CpG methylation
  • DNA methylation
  • DNA methylomics
  • Immunoprecipitation
  • MeDIP
  • MeDIP-on-chip
  • Tiling array
  • Whole genome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hsu, Y. W., Huang, R. L., & Lai, H. C. (2014). MeDIP-on-chip for methylation profiling. In Cervical Cancer: Methods and Protocols (pp. 281-290). Springer New York. https://doi.org/10.1007/978-1-4939-2013-6_21