MeDIP-on-chip for methylation profiling

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

DNA methylation is an important regulatory step in gene expression. Knowledge of the alterations in DNA methylation at a whole genome scale improves our understanding of gene regulation and potential correlations with biological events or disease progression. Methylated DNA immunoprecipitation (MeDIP) uses an antibody that efficiently enriches methylated DNA fragments for downstream locus-specific or genome-wide analyses. MeDIP-on-Chip uses the MeDIP approach in combination with a tiling array for the investigation of genome-wide DNA methylation patterns (or DNA methylomics). The following protocol describes the application of MeDIP to the hybridization of DNA microarrays and data analysis.

Original languageEnglish
Pages (from-to)281-290
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume1249
DOIs
Publication statusPublished - 2015

Fingerprint

Immunoprecipitation
Methylation
DNA
DNA Methylation
Genome
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Disease Progression
Gene Expression
Antibodies
Genes

Keywords

  • 5-Methylcytidine
  • 5-Methylcytidine antibody
  • CpG islands
  • CpG methylation
  • DNA methylation
  • DNA methylomics
  • Immunoprecipitation
  • MeDIP
  • MeDIP-on-Chip
  • Tiling array
  • Whole genome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

MeDIP-on-chip for methylation profiling. / Hsu, Yaw Wen; Huang, Rui-Lan; Lai, Hung Cheng.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1249, 2015, p. 281-290.

Research output: Contribution to journalArticle

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