Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice

Chi Sheng Chiou, Chi Ming Wu, Navneet Kumar Dubey, Wen Cheng Lo, Feng Chou Tsai, Tran Dang Xuan Tung, Wei Ching Hung, Wei Che Hsu, Wei Hong Chen, Win Ping Deng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Osteoarthritis (OA) poses a major clinical challenges owing to limited regenerative ability of diseased or traumatized chondrocytes in articular cartilage. Previous studies have determined the individual therapeutic efficacies of hyaluronic acid (HA) and platelet-rich plasma (PRP) on OA; however, the underlying mechanism is still lacking. Therefore, we investigated mechanistic approach of HA+PRP therapy on chondrocyte apoptosis in IL-1β+TNF-α (I+T) treated in vitro OA model, in addition to in vivo anterior cruciate ligament transection-OA mice model. MTT assay showed an enhanced chondrocyte proliferation and viability in HA+PRP-treated group, compared to I+T, I+T/HA, I+T/PRP, I+T/HA+PRP groups. Further, HA+PRP also significantly suppressed ROS, apoptotic cleaved caspase-3 and PARP, p53 and p21 and MMP-1; whereas, cell cycle modulatory proteins including p-ERK, cyclin B1, D1, and E2 were upregulated. The sub-G1 population and TUNEL assay confirmed the higher abundance of healthy chondrocytes in HA+PRP group. A significantly decreased ARS staining in HA+PRP group was also noted, indicating reduced cartilaginous matrix mineralization compared to other groups. Conclusively, compared to HA or PRP, the combined HA+PRP might be a promising therapy for articular cartilage regeneration in osteoarthritic pathology, possibly via augmented anti-inflammatory, anti-oxidative chondrocyte proliferation and inhibited MMP-1 activity and matrix calcification.

Original languageEnglish
Pages (from-to)4152-4165
Number of pages14
JournalAging
Volume10
Issue number12
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Platelet-Rich Plasma
Hyaluronic Acid
Anti-Inflammatory Agents
Chondrocytes
Interleukin-1
Osteoarthritis
Articular Cartilage
Matrix Metalloproteinases
Cyclin B1
Cell Cycle Proteins
Anterior Cruciate Ligament
Cyclin D1
In Situ Nick-End Labeling
Caspase 3
Regeneration
Therapeutics
Apoptosis
Pathology
Staining and Labeling

Keywords

  • Apoptosis
  • Chondrocyte
  • Hyaluronic acid
  • Inflammation
  • Osteoarthritis
  • Platelet-rich plasma

ASJC Scopus subject areas

  • Ageing
  • Cell Biology

Cite this

Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice. / Chiou, Chi Sheng; Wu, Chi Ming; Dubey, Navneet Kumar; Lo, Wen Cheng; Tsai, Feng Chou; Xuan Tung, Tran Dang; Hung, Wei Ching; Hsu, Wei Che; Chen, Wei Hong; Deng, Win Ping.

In: Aging, Vol. 10, No. 12, 01.12.2018, p. 4152-4165.

Research output: Contribution to journalArticle

Chiou, Chi Sheng ; Wu, Chi Ming ; Dubey, Navneet Kumar ; Lo, Wen Cheng ; Tsai, Feng Chou ; Xuan Tung, Tran Dang ; Hung, Wei Ching ; Hsu, Wei Che ; Chen, Wei Hong ; Deng, Win Ping. / Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice. In: Aging. 2018 ; Vol. 10, No. 12. pp. 4152-4165.
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AU - Deng, Win Ping

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