Mechanistic aspects of CoII(HAPP)(TFA)2 in DNA bulge-specific recognition

Chien Chung Cheng, Wei Chun Huang-Fu, Kuo Chan Hung, Po Jui Chen, Wen Jwu Wang, Yu Ju Chen

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A novel octahedral complex CoII(HAPP)(TFA)2 [hexa-azaphenantholine-cyclophane (HAPP), trifluoroacetate (TFA)] is a DNA bulge-specific probe with single-strand DNA cleavage activity in the presence of H2O2. This complex exhibits low affinity towards double-stranded DNA and low reactivity toward single-stranded DNA. Metal-HAPP complexes with different coordination number and ring size were synthesized and their selectivity and reactivity for DNA bulges were compared. The DNA sequence at the bulge site influences the intensity of cleavage at the bulge and the flanking sites after piperidine treatment. Cleavage specificity of CoII(HAPP)(TFA)2 was characterized extensively using scavenger reagents to quench the cleavage reaction and high-resolution polyacrylamide gel electrophoresis. In addition, 3′-phosphoglycolate cleavage products were trapped and analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. These data were used to deduce that the DNA cleavage pathway for CoIIHAPP2+ in the presence of H2O2 involves 4′-H abstraction of the deoxyribose moiety.

Original languageEnglish
Pages (from-to)2227-2233
Number of pages7
JournalNucleic Acids Research
Volume31
Issue number8
DOIs
Publication statusPublished - Apr 15 2003
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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