Mechanisms of ascorbyl radical formation in human platelet-rich plasma

Kou-Gi Shyu, Chao Chien Chang, Yu Chieh Yeh, Joen Rong Sheu, Duen Suey Chou

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Recently, many clinical reports have suggested that the ascorbyl free radical (Asc) can be treated as a noninvasive, reliable, real-time marker of oxidative stress, but its generation mechanisms in human blood have rarely been discussed. In this study, we used upstream substances, enzyme inhibitors, and free radical scavengers to delineate the mechanisms of Asc formation in human platelet-rich plasma (PRP). Our results show that the doublet signal was detected in PRP samples by using electron spin resonance, and the hyperfine splitting of the doublet signal was aH=1.88 gauss and g-factor = 2.00627, which was determined to be the Asc. We observed that the inhibitors of NADPH oxidase (NOX), cyclooxygenase (COX), lipoxygenase (LOX), cytochrome P450 (CYP450), mitochondria complex III, and nitric oxide synthase (NOS), but not xanthine oxidase, diminished the intensity of the Asc signal dose dependently. All enzyme inhibitors showed no obvious antioxidant activity during a Fenton reaction assay. In summary, the obtained data suggest that Asc formation is associated with NOX, COX, LOX, CYP450, eNOS, and mitochondria in human PRP.

Original languageEnglish
Article number614506
JournalBioMed Research International
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Platelet-Rich Plasma
Platelets
Mitochondria
Lipoxygenase
NADPH Oxidase
Enzyme Inhibitors
Prostaglandin-Endoperoxide Synthases
Plasmas
Cytochrome P-450 Enzyme System
Free Radical Scavengers
Oxidative stress
Xanthine Oxidase
Electron Transport Complex III
Electron Spin Resonance Spectroscopy
Nitric Oxide Synthase
Free Radicals
Paramagnetic resonance
Assays
Oxidative Stress
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Mechanisms of ascorbyl radical formation in human platelet-rich plasma. / Shyu, Kou-Gi; Chang, Chao Chien; Yeh, Yu Chieh; Sheu, Joen Rong; Chou, Duen Suey.

In: BioMed Research International, Vol. 2014, 614506, 2014.

Research output: Contribution to journalArticle

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