Mechanisms involved in the antiplatelet activity of rutin, a glycoside of the flavonol quercetin, in human platelets

Joen Rong Sheu, George Hsiao, Po Hsiu Chou, Ming Yi Shen, Duen Suey Chou

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Abstract

The aim of this study was to systematically examine the inhibitory mechanisms of rutin, a well-known flavonoid in platelet aggregation. In this study, rutin concentration-dependently (250 and 290 μM) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., collagen). Rutin (250 and 290 μM) did not significantly interfere with the binding of FITC-triflavin to the glycoprotein IIb/IIIa complex in human platelets. Rutin (250 and 290 μM) markedly inhibited intracellular Ca2+ mobilization and thromboxane A2 formation in human platelets stimulated by collagen. Rapid phosphorylation of a platelet protein of M r 47000 (P47), a marker of protein kinase C activation, was triggered by collagen (1 μg/mL). This phosphorylation was markedly inhibited by rutin (250 and 290 μM). On the other hand, rutin (250 and 290 μM) did not significantly increase the formations of cyclic AMP and nitric oxide/cyclic GMP in platelets. In conclusion, these results indicate that the antiplatelet activity of rutin may involve the following pathways: rutin inhibited the activation of phospholipase C, followed by inhibition of protein kinase C activity and thromboxane A2 formation, thereby leading to inhibition of the phosphorylation of P47 and intracellular Ca2+ mobilization, finally resulting in inhibition of platelet aggregation.

Original languageEnglish
Pages (from-to)4414-4418
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number14
DOIs
Publication statusPublished - Jul 14 2004

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Rutin
Quercetin
rutin
Glycosides
Platelets
flavonols
quercetin
glycosides
Blood Platelets
Phosphorylation
platelet aggregation
Platelet Aggregation
collagen
phosphorylation
Thromboxane A2
thromboxanes
Collagen
Agglomeration
protein kinase C
Protein Kinase C

Keywords

  • Phospholipase C
  • Platelet aggregation
  • Protein kinase C
  • Rutin
  • Thromboxane A

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

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title = "Mechanisms involved in the antiplatelet activity of rutin, a glycoside of the flavonol quercetin, in human platelets",
abstract = "The aim of this study was to systematically examine the inhibitory mechanisms of rutin, a well-known flavonoid in platelet aggregation. In this study, rutin concentration-dependently (250 and 290 μM) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., collagen). Rutin (250 and 290 μM) did not significantly interfere with the binding of FITC-triflavin to the glycoprotein IIb/IIIa complex in human platelets. Rutin (250 and 290 μM) markedly inhibited intracellular Ca2+ mobilization and thromboxane A2 formation in human platelets stimulated by collagen. Rapid phosphorylation of a platelet protein of M r 47000 (P47), a marker of protein kinase C activation, was triggered by collagen (1 μg/mL). This phosphorylation was markedly inhibited by rutin (250 and 290 μM). On the other hand, rutin (250 and 290 μM) did not significantly increase the formations of cyclic AMP and nitric oxide/cyclic GMP in platelets. In conclusion, these results indicate that the antiplatelet activity of rutin may involve the following pathways: rutin inhibited the activation of phospholipase C, followed by inhibition of protein kinase C activity and thromboxane A2 formation, thereby leading to inhibition of the phosphorylation of P47 and intracellular Ca2+ mobilization, finally resulting in inhibition of platelet aggregation.",
keywords = "Phospholipase C, Platelet aggregation, Protein kinase C, Rutin, Thromboxane A",
author = "Sheu, {Joen Rong} and George Hsiao and Chou, {Po Hsiu} and Shen, {Ming Yi} and Chou, {Duen Suey}",
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T1 - Mechanisms involved in the antiplatelet activity of rutin, a glycoside of the flavonol quercetin, in human platelets

AU - Sheu, Joen Rong

AU - Hsiao, George

AU - Chou, Po Hsiu

AU - Shen, Ming Yi

AU - Chou, Duen Suey

PY - 2004/7/14

Y1 - 2004/7/14

N2 - The aim of this study was to systematically examine the inhibitory mechanisms of rutin, a well-known flavonoid in platelet aggregation. In this study, rutin concentration-dependently (250 and 290 μM) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., collagen). Rutin (250 and 290 μM) did not significantly interfere with the binding of FITC-triflavin to the glycoprotein IIb/IIIa complex in human platelets. Rutin (250 and 290 μM) markedly inhibited intracellular Ca2+ mobilization and thromboxane A2 formation in human platelets stimulated by collagen. Rapid phosphorylation of a platelet protein of M r 47000 (P47), a marker of protein kinase C activation, was triggered by collagen (1 μg/mL). This phosphorylation was markedly inhibited by rutin (250 and 290 μM). On the other hand, rutin (250 and 290 μM) did not significantly increase the formations of cyclic AMP and nitric oxide/cyclic GMP in platelets. In conclusion, these results indicate that the antiplatelet activity of rutin may involve the following pathways: rutin inhibited the activation of phospholipase C, followed by inhibition of protein kinase C activity and thromboxane A2 formation, thereby leading to inhibition of the phosphorylation of P47 and intracellular Ca2+ mobilization, finally resulting in inhibition of platelet aggregation.

AB - The aim of this study was to systematically examine the inhibitory mechanisms of rutin, a well-known flavonoid in platelet aggregation. In this study, rutin concentration-dependently (250 and 290 μM) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., collagen). Rutin (250 and 290 μM) did not significantly interfere with the binding of FITC-triflavin to the glycoprotein IIb/IIIa complex in human platelets. Rutin (250 and 290 μM) markedly inhibited intracellular Ca2+ mobilization and thromboxane A2 formation in human platelets stimulated by collagen. Rapid phosphorylation of a platelet protein of M r 47000 (P47), a marker of protein kinase C activation, was triggered by collagen (1 μg/mL). This phosphorylation was markedly inhibited by rutin (250 and 290 μM). On the other hand, rutin (250 and 290 μM) did not significantly increase the formations of cyclic AMP and nitric oxide/cyclic GMP in platelets. In conclusion, these results indicate that the antiplatelet activity of rutin may involve the following pathways: rutin inhibited the activation of phospholipase C, followed by inhibition of protein kinase C activity and thromboxane A2 formation, thereby leading to inhibition of the phosphorylation of P47 and intracellular Ca2+ mobilization, finally resulting in inhibition of platelet aggregation.

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