Abstract

Stevioside is a sweet-tasting glycoside isolated from the leaves of Stevia rebaudiana. It has been used as a noncaloric sugar substitute in Japan and Brazil for decades. Previous studies have shown that it lowered blood pressure in spontaneously hypertensive rats by intravenous injection. This study was designed to evaluate the hypotensive effect of stevioside in dogs and to define the underlying mechanism. After nasogastric administration of stevioside powder (200 mg/kg), the blood pressure of healthy mongrel dogs began to significantly decrease at 60 min and returned to baseline level at 180 min. The reduction of blood pressure was more rapid (at 5-10 min) and effective after intravenous injection. However, no significant change of blood pressure was noted after injection through left vertebral artery, implicating that the hypotensive effect is not related to the central nervous system. Stevioside also showed significant hypotensive effects in renal hypertensive dogs, in a dose-dependent manner. In cultured rat aortic smooth muscle cells (A7r5 cell line), stevioside can dose-dependently inhibit the stimulatory effects of vasopressin and phenylephrine on intracellular Ca2+ in a calcium-containing medium. However, no intracellular Ca2+ inhibitory effect was observed in calcium-free medium, implicating that stevioside may inhibit the Ca2+ influx from extracellular fluid. Our present data show that stevioside did not influence the calcium ionophore (A23187) induced Ca2+ influx, indicating that the antagonistic effect was through Ca2+ channels. This study confirmed that stevioside is an effective antihypertensive natural product, and its hypotensive mechanism may be probably due to inhibition of the Ca2+ influx.

Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalPharmacology
Volume67
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

Antihypertensive Agents
Dogs
Blood Pressure
Intravenous Injections
Stevia
Calcium
Sweetening Agents
Vertebral Artery
Calcium Ionophores
stevioside
Extracellular Fluid
Calcimycin
Phenylephrine
Inbred SHR Rats
Glycosides
Biological Products
Vasopressins
Powders
Smooth Muscle Myocytes
Brazil

Keywords

  • Anesthetized dogs
  • Hypotension
  • Stevioside

ASJC Scopus subject areas

  • Pharmacology

Cite this

Mechanism of the antihypertensive effect of stevioside in anesthetized dogs. / Liu, Ju Chi; Kao, Pai Keng; Chan, Paul; Hsu, Yung Ho; Hou, Chun-Cheng; Lien, Gi Shih; Hsieh, Min Hsiung; Chen, Yi Jen; Cheng, Juei Tang.

In: Pharmacology, Vol. 67, No. 1, 2003, p. 14-20.

Research output: Contribution to journalArticle

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AU - Kao, Pai Keng

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AU - Lien, Gi Shih

AU - Hsieh, Min Hsiung

AU - Chen, Yi Jen

AU - Cheng, Juei Tang

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