Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts

Yu Lan Yeh, Hsiang I. Tsai, Shiu Min Cheng, Peiying Pai, Tsung Jung Ho, Ray Jade Chen, Chao Hung Lai, Pei Jane Huang, V. Vijaya Padma, Chih Yang Huang

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7 Citations (Scopus)

Abstract

This study investigates the cardio-protective effect of Nos. 1 and 5 extracts from Taiwan Mingjian Oolong Tea on H9c2 cardiomyoblast cells treated with isoproterenol (ISO). Treatment with Nos. 1 and 5 extracts increased cell viability and blocked apoptosis in ISO exposed H9c2 cells. Moreover, Nos. 1 and 5 extracts blocked hypertrophy markers like Gαs, calcineurin, NFATc3, and BNP, thereby increasing cell proliferation markers-PI3K and AKT in a dose dependent manner. In contrast, apoptotic proteins, such as caspase-3 and cytochrome c were decreased in H9c2 cells treated with Nos. 1 and 5 extracts. We confirmed that the protective effect of No. 1 extract was partially mediated through the expression of ERK and p38, however, the No. 5 extract showed a protective effect via the ERK, JNK, and p38 pathways. This evidence provides new insights into the pharmacological role and therapeutic mechanism of Taiwan Mingjian Oolong Tea in heart diseases.

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalAmerican Journal of Chinese Medicine
Volume44
Issue number1
DOIs
Publication statusPublished - Feb 1 2016

Keywords

  • Cardiac Apoptosis
  • Cardiac Hypertrophy
  • Isoproterenol
  • Oolong Tea

ASJC Scopus subject areas

  • Complementary and alternative medicine

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    Yeh, Y. L., Tsai, H. I., Cheng, S. M., Pai, P., Ho, T. J., Chen, R. J., Lai, C. H., Huang, P. J., Padma, V. V., & Huang, C. Y. (2016). Mechanism of Taiwan Mingjian Oolong Tea to Inhibit Isoproterenol-Induced Hypertrophy and Apoptosis in Cardiomyoblasts. American Journal of Chinese Medicine, 44(1), 77-86. https://doi.org/10.1142/S0192415X16500051