Mechanism of nanoformulated graphene oxide-mediated human neutrophil activation

Yu Jen Lu, Yi Hsuan Wang, Rama Shanker Sahu, Jyh Ping Chen, Banendu Sunder Dash, Pei Jen Chung, Hung Wei Yang, Er Yuan Chuang, Tsong Long Hwang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Understanding the molecular mechanisms of graphene oxide (GO)-based biomaterials is important for logical biomedical applications. Previous studies have revealed biointeractions between GO and immune effector cells, but the effects on neutrophils, crucial cells in the immune system, have not been thoroughly discussed. In this study, GO nanoformulations were synthesized with different functional groups, including GO, GO-carboxylated (GO-COOH), and PEGylated GO (GO-PEG), with different surface features, which were elucidated using imaging methods and surface-sensitive quantitative spectroscopic techniques, including atomic force microscopy (AFM), transmission electron microscopy (TEM), and X-ray photoemission spectroscopy (XPS). The GO-based nanoformulations elicited reactive oxygen species (ROS) generation and neutrophil extracellular trap (NET) formation in human neutrophils. Nanoformulated GO stimulates NET development via the formation of ROS. An endocytosis study revealed that nanoformulated GO facilitated internalization by neutrophils via macropinocytosis and actin-dependent phagocytosis. Importantly, calcium mobilization and phosphorylation proteins such as mitogen-Activated protein kinases (extracellular signal-regulated kinase, c-Jun N-Terminal kinase, and p38) and AKT were involved in the activation of neutrophils. These findings offer the first verification that nanoformulated GO exhibits direct effects on human neutrophils.

Original languageEnglish
Pages (from-to)40141-40152
Number of pages12
JournalACS Applied Materials and Interfaces
Volume12
Issue number36
DOIs
Publication statusPublished - Sep 9 2020

Keywords

  • CD11b
  • graphene oxide
  • neutrophil
  • neutrophil extracellular trap
  • reactive oxygen species

ASJC Scopus subject areas

  • Materials Science(all)

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