Mechanism of c-erbB transduction

Newly released transducing viruses retain poly(A) tracts of erbB transcripts and encode C-terminally intact erbB proteins

M. A. Raines, N. J. Maihle, C. Moscovici, L. Crittenden, K. J. Kung

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have previously shown that avian leukosis virus (ALV) induces erythroblastosis by insertional activation of the c-erbB gene. In 25% of the ALV-induced leukemic samples we have analyzed, acute retroviruses that have captured the activated erbB oncogene were released. The unusually high frequency at which erbB transduction occurs makes this an ideal system for studying the mechanism of oncogene transduction. In addition, these leukemic samples provide a rich source for the isolation of novel erbB-transducing viruses. We report here our characterization of several new erbB-transducing proviruses. The 5' recombinant points of all these viruses mapped to the same intron in which proviral insertions cluster, supporting the hypothesis that transduction begins with proviral insertion near the oncogene. The 3' recombination points usually occurred within the 3' untranslated region downstream from the termination codon of the c-erbB gene. Three of the erbB-containing proviruses were molecularly cloned and analyzed in detail. Two of them were capable of releasing acute viruses, and interestingly, both retained poly(A) tracts of erbB messages in their genomes. A stretch of six adenosine residues in the ALV env gene appeared to mediate the 3' recombination events required for the generation of these viruses. These data provide further insight into the mechanism by which oncogenes are transduced into retroviral genomes.

Original languageEnglish
Pages (from-to)2437-2443
Number of pages7
JournalJournal of Virology
Volume62
Issue number7
Publication statusPublished - Jan 1 1988
Externally publishedYes

Fingerprint

Poly A
oncogenes
Avian Leukosis Virus
Avian leukosis virus
Oncogenes
erbB Genes
Viruses
proviruses
Proviruses
viruses
Genetic Recombination
Proteins
Retroviridae
proteins
Genome
env Genes
genome
genes
Terminator Codon
stop codon

ASJC Scopus subject areas

  • Immunology

Cite this

Mechanism of c-erbB transduction : Newly released transducing viruses retain poly(A) tracts of erbB transcripts and encode C-terminally intact erbB proteins. / Raines, M. A.; Maihle, N. J.; Moscovici, C.; Crittenden, L.; Kung, K. J.

In: Journal of Virology, Vol. 62, No. 7, 01.01.1988, p. 2437-2443.

Research output: Contribution to journalArticle

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