Mechanical coupling of cytoskeletal elasticity and force generation is crucial for understanding the migrating nature of keloid fibroblasts

Hans I Chen Harn, Yang Kao Wang, Chao Kai Hsu, Yen Ting Ho, Yi Wei Huang, Wen Tai Chiu, Hsi Hui Lin, Chao Min Cheng, Ming Jer Tang

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    One of the key features of keloid is its fibroblasts migrating beyond the original wound border. During migration, cells not only undergo molecular changes but also mechanical modulation. This process is led by actin filaments serving as the backbone of intra-cellular force and transduces external mechanical signal via focal adhesion complex into the cell. Here, we focus on determining the mechanical changes of actin filaments and the spatial distribution of forces in response to changing chemical stimulations and during cell migration. Atomic force microscopy and micropost array detector are used to determine and compare the magnitude and distribution of filament elasticity and force generation in fibroblasts and keloid fibroblasts. We found both filament elasticity and force generation show spatial distribution in a polarized and migrating cell. Such spatial distribution is disrupted when mechano-signalling is perturbed by focal adhesion kinase inhibitor and in keloid fibroblasts. The demonstration of keloid pathology at the nanoscale highlights the coupling of cytoskeletal function with physical characters at the subcellular level and provides new research directions for migration-related disease such as keloid.

    Original languageEnglish
    Pages (from-to)579-584
    Number of pages6
    JournalExperimental Dermatology
    Volume24
    Issue number8
    DOIs
    Publication statusPublished - Aug 1 2015

    Fingerprint

    Keloid
    Elasticity
    Fibroblasts
    Spatial distribution
    Actins
    Actin Cytoskeleton
    Focal Adhesion Protein-Tyrosine Kinases
    Chemical Stimulation
    Pathology
    Focal Adhesions
    Atomic Force Microscopy
    Atomic force microscopy
    Demonstrations
    Adhesion
    Modulation
    Cell Movement
    Detectors
    Wounds and Injuries
    Research

    Keywords

    • Actin
    • Atomic force microscopy
    • Force
    • Keloid
    • Migration

    ASJC Scopus subject areas

    • Dermatology
    • Molecular Biology
    • Biochemistry
    • Medicine(all)

    Cite this

    Mechanical coupling of cytoskeletal elasticity and force generation is crucial for understanding the migrating nature of keloid fibroblasts. / Harn, Hans I Chen; Wang, Yang Kao; Hsu, Chao Kai; Ho, Yen Ting; Huang, Yi Wei; Chiu, Wen Tai; Lin, Hsi Hui; Cheng, Chao Min; Tang, Ming Jer.

    In: Experimental Dermatology, Vol. 24, No. 8, 01.08.2015, p. 579-584.

    Research output: Contribution to journalArticle

    Harn, Hans I Chen ; Wang, Yang Kao ; Hsu, Chao Kai ; Ho, Yen Ting ; Huang, Yi Wei ; Chiu, Wen Tai ; Lin, Hsi Hui ; Cheng, Chao Min ; Tang, Ming Jer. / Mechanical coupling of cytoskeletal elasticity and force generation is crucial for understanding the migrating nature of keloid fibroblasts. In: Experimental Dermatology. 2015 ; Vol. 24, No. 8. pp. 579-584.
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