Mechamism of action of a potent antiplatelet peptide, triflavin from Trimeresurus flavoviridis snake venom

T. F. Huang, J. R. Sheu, C. M. Teng

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Triflavin, an antiplatelet peptide from Trimeresurus flavoviridis snake venom, inhibits aggregation of human platelets stimulated by a variety of agonists. However, triflavin does not affect the shape change and release reaction of platelets stimulated by thrombin and collagen. In this paper, we further investigate its effect on the intracellular events occurring after the activation of platelets. Triflavin does not inhibit the intracellular free calcium rise of Quin 2-AM loaded platelets stimulated by thrombin and it also has no significant effect on thromboxane B2 formation of platelets stimulated by thrombin. Triflavin does not affect the 3(H)-inositol monophosphate formation of the 3(H)myoinositol loaded platelets. However, triflavin dose-dependently inhibits fibrinogen-induced aggregation and 125I-fibrinogen binding of ADP-stimulated platelets. In addition, triflavin dose-dependently blocks fibrinogen-induced aggregation of elastase-treated platelets. It is concluded that trifavin specifically inhibits fibrinogen binding to fibrinogen receptors associated with glycoprotein IIb/IIIa complex on platelet membrane surface without any inhibitory effect on the platelet-activation process.

Original languageEnglish
Pages (from-to)489-493
Number of pages5
JournalThrombosis and Haemostasis
Volume66
Issue number4
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Trimeresurus
Snake Venoms
Blood Platelets
Peptides
Fibrinogen
Thrombin
Platelet Activation
Inositol
Fibrinogen Receptors
Thromboxane B2
Platelet Glycoprotein GPIIb-IIIa Complex
Pancreatic Elastase
triflavin
Platelet Aggregation
Adenosine Diphosphate
Collagen
Calcium
Membranes

ASJC Scopus subject areas

  • Hematology

Cite this

Mechamism of action of a potent antiplatelet peptide, triflavin from Trimeresurus flavoviridis snake venom. / Huang, T. F.; Sheu, J. R.; Teng, C. M.

In: Thrombosis and Haemostasis, Vol. 66, No. 4, 1991, p. 489-493.

Research output: Contribution to journalArticle

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