Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry

Yu Min Lin, Der Zen Liu, Haw Ming Haw, Li Ping Tseng

Research output: Contribution to journalArticle

12 Citations (Scopus)


In this study, we employed a thermodynamic method to obtain information on the second virial coefficient of the interactions between liposomes, and we also determined the stabilities of dipalmitoyl-L-alpha-phosphatidylglycerol (DPPG)- and dipalmitoyl-L-alpha-phosphatidylcholine (DPPC)-liposomes. In this study, an interaction model based on a hard-sphere interaction potential assumption was employed, and we used high-sensitivity isothermal titration nanocalorimetry (ITC) to measure the dilution heat of liposomal suspensions. Information on the second virial coefficient of interactions between liposomes can be derived from the number density of the liposomal suspension. Additionally, we qualitatively demonstrated the repulsive forces of DPPG- and DPPC-liposomes systems. In this work, both the b'2 and b2/B0 levels of DPPG-liposome were greater and had better physical stability than those of DPPC-liposome.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalJournal of the Chinese Institute of Chemical Engineers
Issue number2
Publication statusPublished - Mar 2007



  • Interaction potential
  • ITC
  • Liposome
  • Second virial coefficient

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this