Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry

Yu Min Lin, Der Zen Liu, Haw Ming Haw, Li Ping Tseng

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we employed a thermodynamic method to obtain information on the second virial coefficient of the interactions between liposomes, and we also determined the stabilities of dipalmitoyl-L-alpha-phosphatidylglycerol (DPPG)- and dipalmitoyl-L-alpha-phosphatidylcholine (DPPC)-liposomes. In this study, an interaction model based on a hard-sphere interaction potential assumption was employed, and we used high-sensitivity isothermal titration nanocalorimetry (ITC) to measure the dilution heat of liposomal suspensions. Information on the second virial coefficient of interactions between liposomes can be derived from the number density of the liposomal suspension. Additionally, we qualitatively demonstrated the repulsive forces of DPPG- and DPPC-liposomes systems. In this work, both the b'2 and b2/B0 levels of DPPG-liposome were greater and had better physical stability than those of DPPC-liposome.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalJournal of the Chinese Institute of Chemical Engineers
Volume38
Issue number2
DOIs
Publication statusPublished - Mar 2007

Fingerprint

1,2-Dipalmitoylphosphatidylcholine
Phosphatidylglycerols
Liposomes
Calorimetry
Titration
Phosphatidylcholines
Suspensions
Dilution
Thermodynamics

Keywords

  • Interaction potential
  • ITC
  • Liposome
  • Second virial coefficient

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

@article{5d4da9690cae4c638c402ca36176c7c6,
title = "Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry",
abstract = "In this study, we employed a thermodynamic method to obtain information on the second virial coefficient of the interactions between liposomes, and we also determined the stabilities of dipalmitoyl-L-alpha-phosphatidylglycerol (DPPG)- and dipalmitoyl-L-alpha-phosphatidylcholine (DPPC)-liposomes. In this study, an interaction model based on a hard-sphere interaction potential assumption was employed, and we used high-sensitivity isothermal titration nanocalorimetry (ITC) to measure the dilution heat of liposomal suspensions. Information on the second virial coefficient of interactions between liposomes can be derived from the number density of the liposomal suspension. Additionally, we qualitatively demonstrated the repulsive forces of DPPG- and DPPC-liposomes systems. In this work, both the b'2 and b2/B0 levels of DPPG-liposome were greater and had better physical stability than those of DPPC-liposome.",
keywords = "Interaction potential, ITC, Liposome, Second virial coefficient",
author = "Lin, {Yu Min} and Liu, {Der Zen} and Haw, {Haw Ming} and Tseng, {Li Ping}",
year = "2007",
month = "3",
doi = "10.1016/j.jcice.2006.11.002",
language = "English",
volume = "38",
pages = "103--106",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
issn = "1876-1070",
publisher = "Taiwan Institute of Chemical Engineers",
number = "2",

}

TY - JOUR

T1 - Measurement of the second virial coefficient of DPPC- and DPPG-liposomes by isothermal titration calorimetry

AU - Lin, Yu Min

AU - Liu, Der Zen

AU - Haw, Haw Ming

AU - Tseng, Li Ping

PY - 2007/3

Y1 - 2007/3

N2 - In this study, we employed a thermodynamic method to obtain information on the second virial coefficient of the interactions between liposomes, and we also determined the stabilities of dipalmitoyl-L-alpha-phosphatidylglycerol (DPPG)- and dipalmitoyl-L-alpha-phosphatidylcholine (DPPC)-liposomes. In this study, an interaction model based on a hard-sphere interaction potential assumption was employed, and we used high-sensitivity isothermal titration nanocalorimetry (ITC) to measure the dilution heat of liposomal suspensions. Information on the second virial coefficient of interactions between liposomes can be derived from the number density of the liposomal suspension. Additionally, we qualitatively demonstrated the repulsive forces of DPPG- and DPPC-liposomes systems. In this work, both the b'2 and b2/B0 levels of DPPG-liposome were greater and had better physical stability than those of DPPC-liposome.

AB - In this study, we employed a thermodynamic method to obtain information on the second virial coefficient of the interactions between liposomes, and we also determined the stabilities of dipalmitoyl-L-alpha-phosphatidylglycerol (DPPG)- and dipalmitoyl-L-alpha-phosphatidylcholine (DPPC)-liposomes. In this study, an interaction model based on a hard-sphere interaction potential assumption was employed, and we used high-sensitivity isothermal titration nanocalorimetry (ITC) to measure the dilution heat of liposomal suspensions. Information on the second virial coefficient of interactions between liposomes can be derived from the number density of the liposomal suspension. Additionally, we qualitatively demonstrated the repulsive forces of DPPG- and DPPC-liposomes systems. In this work, both the b'2 and b2/B0 levels of DPPG-liposome were greater and had better physical stability than those of DPPC-liposome.

KW - Interaction potential

KW - ITC

KW - Liposome

KW - Second virial coefficient

UR - http://www.scopus.com/inward/record.url?scp=38549146471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38549146471&partnerID=8YFLogxK

U2 - 10.1016/j.jcice.2006.11.002

DO - 10.1016/j.jcice.2006.11.002

M3 - Article

VL - 38

SP - 103

EP - 106

JO - Journal of the Taiwan Institute of Chemical Engineers

JF - Journal of the Taiwan Institute of Chemical Engineers

SN - 1876-1070

IS - 2

ER -