Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.

C. C. Shieh, S. C. Chang, C. R. Tzeng, J. J. Huang, W. J. Nir, C. Y. Hong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.

Original languageEnglish
Pages (from-to)614-619
Number of pages6
JournalAndrologia
Volume19
Issue number6
Publication statusPublished - Nov 1987
Externally publishedYes

Fingerprint

Semen
Saliva
Testosterone
Serum
Sperm Motility
Sepharose
Antibodies

ASJC Scopus subject areas

  • Endocrinology
  • Nephrology

Cite this

Shieh, C. C., Chang, S. C., Tzeng, C. R., Huang, J. J., Nir, W. J., & Hong, C. Y. (1987). Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay. Andrologia, 19(6), 614-619.

Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay. / Shieh, C. C.; Chang, S. C.; Tzeng, C. R.; Huang, J. J.; Nir, W. J.; Hong, C. Y.

In: Andrologia, Vol. 19, No. 6, 11.1987, p. 614-619.

Research output: Contribution to journalArticle

Shieh, CC, Chang, SC, Tzeng, CR, Huang, JJ, Nir, WJ & Hong, CY 1987, 'Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.', Andrologia, vol. 19, no. 6, pp. 614-619.
Shieh, C. C. ; Chang, S. C. ; Tzeng, C. R. ; Huang, J. J. ; Nir, W. J. ; Hong, C. Y. / Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay. In: Andrologia. 1987 ; Vol. 19, No. 6. pp. 614-619.
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