Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.

C. C. Shieh, S. C. Chang, C. R. Tzeng, J. J. Huang, W. J. Nir, C. Y. Hong

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.

Original languageEnglish
Pages (from-to)614-619
Number of pages6
JournalAndrologia
Volume19
Issue number6
Publication statusPublished - Nov 1987
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology
  • Nephrology

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