MCPIP1 ribonuclease exhibits broad-spectrum antiviral effects through viral RNA binding and degradation

Ren-Jye Lin, Hsu Ling Chien, Shyr Yi Lin, Bi Lan Chang, Han Pang Yu, Wei Chun Tang, Yi Ling Lin

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Monocyte chemoattractant protein 1-induced protein 1 (MCPIP1), belonging to the MCPIP family with highly conserved CCCH-type zinc finger and Nedd4-BP1, YacP Nuclease domains, has been implicated in negative regulation of the cellular inflammatory responses. In this report, we demonstrate for the first time that this RNA-binding nuclease also targets viral RNA and possesses potent antiviral activities. Overexpression of the human MCPIP1, but not MCPIP2, MCPIP3 or MCPIP4, inhibited Japanese encephalitis virus (JEV) and dengue virus (DEN) replication. The functional analysis of MCPIP1 revealed that the activities of RNase, RNA binding and oligomerization, but not deubiqutinase, are required for its antiviral potential. Furthermore, infection of other positive-sense RNA viruses, such as sindbis virus and encephalomyocarditis virus, and negative-sense RNA virus, such as influenza virus, as well as DNA virus, such as adenovirus, can also be blocked by MCPIP1. Moreover, the endogenous MCPIP1 gene expression was induced by JEV and DEN infection, and knockdown of MCPIP1 expression enhanced the replication of JEV and DEN in human cells. Thus, MCPIP1 can act as a host innate defense via RNase activity for targeting and degrading viral RNA.

Original languageEnglish
Pages (from-to)3314-3326
Number of pages13
JournalNucleic Acids Research
Volume41
Issue number5
DOIs
Publication statusPublished - Mar 2013

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Chemokine CCL2
RNA Stability
Viral RNA
Ribonucleases
Antiviral Agents
Japanese Encephalitis Virus
Dengue Virus
Proteins
RNA Viruses
Encephalomyocarditis virus
Sindbis Virus
DNA Viruses
Zinc Fingers
Virus Diseases
Virus Replication
Orthomyxoviridae
Adenoviridae
RNA
Gene Expression
Infection

ASJC Scopus subject areas

  • Genetics

Cite this

MCPIP1 ribonuclease exhibits broad-spectrum antiviral effects through viral RNA binding and degradation. / Lin, Ren-Jye; Chien, Hsu Ling; Lin, Shyr Yi; Chang, Bi Lan; Yu, Han Pang; Tang, Wei Chun; Lin, Yi Ling.

In: Nucleic Acids Research, Vol. 41, No. 5, 03.2013, p. 3314-3326.

Research output: Contribution to journalArticle

Lin, Ren-Jye ; Chien, Hsu Ling ; Lin, Shyr Yi ; Chang, Bi Lan ; Yu, Han Pang ; Tang, Wei Chun ; Lin, Yi Ling. / MCPIP1 ribonuclease exhibits broad-spectrum antiviral effects through viral RNA binding and degradation. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 5. pp. 3314-3326.
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