MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer

Nicole Dodge Zantek, Jennifer Walker-Daniels, Jane Stewart, Rhonda K. Hansen, Daniel Robinson, Hui Miao, Bingcheng Wang, Hsing Jien Kung, Mina J. Bissell, Michael S. Kinch

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Abstract

Purpose: There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. Experimental Design: We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. Results: NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. Conclusions: MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.

Original languageEnglish
Pages (from-to)3640-3648
Number of pages9
JournalClinical Cancer Research
Volume7
Issue number11
Publication statusPublished - Jan 1 2001
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Dodge Zantek, N., Walker-Daniels, J., Stewart, J., Hansen, R. K., Robinson, D., Miao, H., Wang, B., Kung, H. J., Bissell, M. J., & Kinch, M. S. (2001). MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer. Clinical Cancer Research, 7(11), 3640-3648.