MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2

Kai Wen Hsu, Rong Hong Hsieh, Chew Wun Wu, Chin Wen Chi, Yan Hwa Wu Lee, Min Liang Kuo, Kou Juey Wu, Tian-Shun Tsai

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The c-Myc promoter binding protein 1 (MBP-1) is a transcriptional suppressor of c-myc expression and involved in control of tumorigenesis. Gastric cancer is one of the most frequent neoplasms and lethal malignancies worldwide. So far, the regulatory mechanism of its aggressiveness has not been clearly characterized. Here we studied roles of MBP-1 in gastric cancer progression. We found that cell proliferation was inhibited by MBP-1 overexpression in human stomach adenocarcinoma SC-M1 cells. Colony formation, migration, and invasion abilities of SC-M1 cells were suppressed by MBP-1 overexpression but promoted by MBP-1 knockdown. Furthermore, the xenografted tumor growth of SC-M1 cells was suppressed by MBP-1 overexpression. Metastasis in lungs of mice was inhibited by MBP-1 after tail vein injection with SC-M1 cells. MBP-1 also suppressed epithelial-mesenchymal transition in SC-M1 cells. Additionally, MBP-1 bound on cyclooxygenase 2 (COX-2) promoter and downregulated COX-2 expression. The MBP-1-suppressed tumor progression in SC-M1 cells were through inhibition of COX-2 expression. MBP-1 also exerted a suppressive effect on tumor progression of other gastric cancer cells such as AGS and NUGC-3 cells. Taken together, these results suggest that MBP-1-suppressed COX-2 expression plays an important role in the inhibition of growth and progression of gastric cancer.

Original languageEnglish
Pages (from-to)5127-5137
Number of pages11
JournalMolecular Biology of the Cell
Volume20
Issue number24
DOIs
Publication statusPublished - 2009

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Cyclooxygenase 2
Stomach Neoplasms
Carrier Proteins
Neoplasm Metastasis
Growth
Neoplasms
Epithelial-Mesenchymal Transition
Tail
Veins
Stomach
Carcinogenesis
Adenocarcinoma
Down-Regulation
Cell Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Hsu, K. W., Hsieh, R. H., Wu, C. W., Chi, C. W., Lee, Y. H. W., Kuo, M. L., ... Tsai, T-S. (2009). MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2. Molecular Biology of the Cell, 20(24), 5127-5137. https://doi.org/10.1091/mbc.E09-05-0386

MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2. / Hsu, Kai Wen; Hsieh, Rong Hong; Wu, Chew Wun; Chi, Chin Wen; Lee, Yan Hwa Wu; Kuo, Min Liang; Wu, Kou Juey; Tsai, Tian-Shun.

In: Molecular Biology of the Cell, Vol. 20, No. 24, 2009, p. 5127-5137.

Research output: Contribution to journalArticle

Hsu, KW, Hsieh, RH, Wu, CW, Chi, CW, Lee, YHW, Kuo, ML, Wu, KJ & Tsai, T-S 2009, 'MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2', Molecular Biology of the Cell, vol. 20, no. 24, pp. 5127-5137. https://doi.org/10.1091/mbc.E09-05-0386
Hsu, Kai Wen ; Hsieh, Rong Hong ; Wu, Chew Wun ; Chi, Chin Wen ; Lee, Yan Hwa Wu ; Kuo, Min Liang ; Wu, Kou Juey ; Tsai, Tian-Shun. / MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 24. pp. 5127-5137.
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