Matrix metalloproteinase-9 is essential for ischemia-induced neovascularization by modulating bone marrow-derived endothelial progenitor Cells

Po Hsun Huang, Yung Hsiang Chen, Chao Hung Wang, Jia Shiong Chen, Hsiao Ya Tsai, Feng Yen Lin, Wei Yuh Lo, Tao Cheng Wu, Masataka Sata, Jaw Wen Chen, Shing Jong Lin

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

OBJECTIVE-: Both matrix metalloproteinases (MMPs) and endothelial progenitor cells (EPCs) have been implicated in the process of neovascularization. Here we show that the impaired neovascularization in mice lacking MMP-9 is related to a defect in EPC functions in vasculogenesis. METHODS AND RESULTS-: Hindlimb ischemia surgery was conducted in MMP-9 mice and wild-type (MMP-9) mice. Blood flow recovery was markedly impaired in MMP-9 mice when compared with that in wild-type mice as determined by laser Doppler imaging. Flow cytometry demonstrated that the number of EPC-like cells (Sca-1/Flk-1) in peripheral blood increased in wild-type mice after hindlimb ischemia surgery and exogenous vascular endothelial growth factor stimulation, but not in MMP-9 mice. Plasma levels and bone marrow concentrations of soluble Kit-ligand (sKitL) were significantly elevated in wild-type mice in response to tissue ischemia, but not in MMP-9 mice. C-kit positive bone marrow cells of MMP-9 mice have attenuated adhesion and migration than those isolated from wild-type mice. In in vitro studies, incubation with selective MMP-9 inhibitor suppressed the colony formation, migration, and tube formation capacities of EPC. Transplantation of bone marrow cells from wild-type mice restored collateral flow formation in MMP-9 mice. CONCLUSIONS-: These findings suggest that MMP-9 deficiency impairs ischemia-induced neovascularization, and these effects may occur through a reduction in releasing the stem cell-active cytokine, and EPC mobilization, migration, and vasculogenesis functions.

Original languageEnglish
Pages (from-to)1179-1184
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume29
Issue number8
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Matrix Metalloproteinase 9
Ischemia
Bone Marrow
Hindlimb
Endothelial Progenitor Cells
Stem Cell Factor
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinases
Bone Marrow Transplantation
Bone Marrow Cells
Vascular Endothelial Growth Factor A
Cell Movement
Flow Cytometry
Lasers
Stem Cells

Keywords

  • Endothelial progenitor cells
  • Matrix metalloproteinases
  • Neovascularization

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Matrix metalloproteinase-9 is essential for ischemia-induced neovascularization by modulating bone marrow-derived endothelial progenitor Cells. / Huang, Po Hsun; Chen, Yung Hsiang; Wang, Chao Hung; Chen, Jia Shiong; Tsai, Hsiao Ya; Lin, Feng Yen; Lo, Wei Yuh; Wu, Tao Cheng; Sata, Masataka; Chen, Jaw Wen; Lin, Shing Jong.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 29, No. 8, 08.2009, p. 1179-1184.

Research output: Contribution to journalArticle

Huang, Po Hsun ; Chen, Yung Hsiang ; Wang, Chao Hung ; Chen, Jia Shiong ; Tsai, Hsiao Ya ; Lin, Feng Yen ; Lo, Wei Yuh ; Wu, Tao Cheng ; Sata, Masataka ; Chen, Jaw Wen ; Lin, Shing Jong. / Matrix metalloproteinase-9 is essential for ischemia-induced neovascularization by modulating bone marrow-derived endothelial progenitor Cells. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2009 ; Vol. 29, No. 8. pp. 1179-1184.
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T1 - Matrix metalloproteinase-9 is essential for ischemia-induced neovascularization by modulating bone marrow-derived endothelial progenitor Cells

AU - Huang, Po Hsun

AU - Chen, Yung Hsiang

AU - Wang, Chao Hung

AU - Chen, Jia Shiong

AU - Tsai, Hsiao Ya

AU - Lin, Feng Yen

AU - Lo, Wei Yuh

AU - Wu, Tao Cheng

AU - Sata, Masataka

AU - Chen, Jaw Wen

AU - Lin, Shing Jong

PY - 2009/8

Y1 - 2009/8

N2 - OBJECTIVE-: Both matrix metalloproteinases (MMPs) and endothelial progenitor cells (EPCs) have been implicated in the process of neovascularization. Here we show that the impaired neovascularization in mice lacking MMP-9 is related to a defect in EPC functions in vasculogenesis. METHODS AND RESULTS-: Hindlimb ischemia surgery was conducted in MMP-9 mice and wild-type (MMP-9) mice. Blood flow recovery was markedly impaired in MMP-9 mice when compared with that in wild-type mice as determined by laser Doppler imaging. Flow cytometry demonstrated that the number of EPC-like cells (Sca-1/Flk-1) in peripheral blood increased in wild-type mice after hindlimb ischemia surgery and exogenous vascular endothelial growth factor stimulation, but not in MMP-9 mice. Plasma levels and bone marrow concentrations of soluble Kit-ligand (sKitL) were significantly elevated in wild-type mice in response to tissue ischemia, but not in MMP-9 mice. C-kit positive bone marrow cells of MMP-9 mice have attenuated adhesion and migration than those isolated from wild-type mice. In in vitro studies, incubation with selective MMP-9 inhibitor suppressed the colony formation, migration, and tube formation capacities of EPC. Transplantation of bone marrow cells from wild-type mice restored collateral flow formation in MMP-9 mice. CONCLUSIONS-: These findings suggest that MMP-9 deficiency impairs ischemia-induced neovascularization, and these effects may occur through a reduction in releasing the stem cell-active cytokine, and EPC mobilization, migration, and vasculogenesis functions.

AB - OBJECTIVE-: Both matrix metalloproteinases (MMPs) and endothelial progenitor cells (EPCs) have been implicated in the process of neovascularization. Here we show that the impaired neovascularization in mice lacking MMP-9 is related to a defect in EPC functions in vasculogenesis. METHODS AND RESULTS-: Hindlimb ischemia surgery was conducted in MMP-9 mice and wild-type (MMP-9) mice. Blood flow recovery was markedly impaired in MMP-9 mice when compared with that in wild-type mice as determined by laser Doppler imaging. Flow cytometry demonstrated that the number of EPC-like cells (Sca-1/Flk-1) in peripheral blood increased in wild-type mice after hindlimb ischemia surgery and exogenous vascular endothelial growth factor stimulation, but not in MMP-9 mice. Plasma levels and bone marrow concentrations of soluble Kit-ligand (sKitL) were significantly elevated in wild-type mice in response to tissue ischemia, but not in MMP-9 mice. C-kit positive bone marrow cells of MMP-9 mice have attenuated adhesion and migration than those isolated from wild-type mice. In in vitro studies, incubation with selective MMP-9 inhibitor suppressed the colony formation, migration, and tube formation capacities of EPC. Transplantation of bone marrow cells from wild-type mice restored collateral flow formation in MMP-9 mice. CONCLUSIONS-: These findings suggest that MMP-9 deficiency impairs ischemia-induced neovascularization, and these effects may occur through a reduction in releasing the stem cell-active cytokine, and EPC mobilization, migration, and vasculogenesis functions.

KW - Endothelial progenitor cells

KW - Matrix metalloproteinases

KW - Neovascularization

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