Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice

Chian Jiun Liou, You Rong Lai, Ya Ling Chen, Yi Hsien Chang, Zih Ying Li, Wen Chung Huang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Matrine is isolated from Sophora flavescens and shows anti-inflammatory effects in macrophages. Here we evaluated matrine's suppressive effects on cyclooxygenase 2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expressions in lipopolysaccharide-(LPS-) stimulated human lung epithelial A549 cells. Additionally, BALB/c mice were given various matrine doses by intraperitoneal injection, and then lung injury was induced via intratracheal instillation of LPS. In LPS-stimulated A549 cells, matrine inhibited the productions of interleukin-8 (IL-8), monocyte chemotactic protein-1, and IL-6 and decreased COX-2 expression. Matrine treatment also decreased ICAM-1 protein expression and suppressed the adhesion of neutrophil-like cells to inflammatory A549 cells. In vitro results demonstrated that matrine significantly inhibited mitogen-activated protein kinase phosphorylation and decreased nuclear transcription factor kappa-B subunit p65 protein translocation into the nucleus. In vivo data indicated that matrine significantly inhibited neutrophil infiltration and suppressed productions of tumor necrosis factor-α and IL-6 in mouse bronchoalveolar lavage fluid and serum. Analysis of lung tissue showed that matrine decreased the gene expression of proinflammatory cytokines, chemokines, COX-2, and ICAM-1. Our findings suggest that matrine improved lung injury in mice and decreased the inflammatory response in human lung epithelial cells.

Original languageEnglish
Article number3630485
JournalMediators of Inflammation
Volume2016
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

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Acute Lung Injury
Intercellular Adhesion Molecule-1
Cyclooxygenase 2
Epithelial Cells
Lung
Lung Injury
Sophora
matrine
Neutrophil Infiltration
NF-kappa B
Chemokine CCL2
Bronchoalveolar Lavage Fluid
Protein Transport
Mitogen-Activated Protein Kinases
Intraperitoneal Injections
Interleukin-8
Chemokines
Lipopolysaccharides
Interleukin-6
Neutrophils

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Matrine Attenuates COX-2 and ICAM-1 Expressions in Human Lung Epithelial Cells and Prevents Acute Lung Injury in LPS-Induced Mice. / Liou, Chian Jiun; Lai, You Rong; Chen, Ya Ling; Chang, Yi Hsien; Li, Zih Ying; Huang, Wen Chung.

In: Mediators of Inflammation, Vol. 2016, 3630485, 01.01.2016.

Research output: Contribution to journalArticle

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