Background:Maternal tobacco smoke exposure adversely affected fetal kidney development. Nicotine stimulates epithelial-mesenchymal transition and connective tissue growth factor (CTGF) expression in the renal epithelium. We hypothesized that maternal nicotine exposure would induce kidney fibrosis and involve CTGF in newborn rats.Methods:Nicotine was administered to pregnant Sprague-Dawley rats at a dose of 6 mg/kg/d from gestational days 7-21 and gestational day 7 to postnatal day 14. A control group was injected with normal saline. Neonatal kidney tissues underwent histological analysis, collagen measurement, and western blot analysis.Results:Tubular injury scores and total collagen contents were significantly higher in rats born to nicotine-treated dams than in rats born to normal saline-treated dams on postnatal days 7 and 21. Masson's trichrome staining further verified the presence of kidney fibrosis. Prenatal and/or postnatal nicotine exposure increased CTGF expression on postnatal days 7 and 21.Conclusion:Maternal nicotine exposure during gestation and lactation induces neonatal kidney fibrosis, and CTGF may be involved in the pathogenesis of kidney fibrosis. These results may be relevant to premature low-birth-weight infants who are conveyed a high risk of developing chronic kidney disease and exposed to breast milk of smoking mothers during the neonatal period.
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health