Maternal Nicotine Effects on Lung Collagen Gene Expression in Newborn Rats

Chung Ming Chen, Leng-Fang Wang, Borcherng Su

Research output: Contribution to journalArticle

Abstract

We evaluated the effects of maternal nicotine treatment on collagen gene expression in newborn rat lungs. Timed pregnant Sprague-Dawley rats were injected subcutaneously with nicotine tartrate (2 mg/kg/day) from day 3 to day 21 of gestation. A control group was injected with an equal volume of 0.9% NaCl. On days 1, 7, 14, and 21 after birth, rat pups were randomly selected from each group and lungs were removed for measurement of collagen gene expression and collagen contents by reverse transcription-polymerase chain reaction and histology, respectively. Body weights of nicotine-treated dams were lower than those of control dams from gestational days 5 to 21, and the values reached statistical significance on gestational days 17, 20, and 21. The body weight, lung weight, and lung/body weight ratio were comparable between control and nicotine-exposed rats. Lung collagen I and III mRNA expressions and collagen-staining pattern in alveolar septa were similar between control and nicotine-exposed rats during the study period. We conclude that maternal exposure to nicotine (2 mg/kg/Day) during pregnancy does not influence collagen gene expression or collagen contents in postnatal rat lungs.

Original languageEnglish
Pages (from-to)327-331
Number of pages5
JournalActa Paediatrica Taiwanica
Volume44
Issue number6
Publication statusPublished - Nov 2003

Fingerprint

Nicotine
Collagen
Gene Expression
Lung
Body Weight
Maternal Exposure
Pregnancy
Maternal Inheritance
Reverse Transcription
Sprague Dawley Rats
Histology
Mothers
Parturition
Staining and Labeling
Weights and Measures
Polymerase Chain Reaction
Control Groups
Messenger RNA

Keywords

  • Collagen
  • Lung
  • Nicotine
  • Rat

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Maternal Nicotine Effects on Lung Collagen Gene Expression in Newborn Rats. / Chen, Chung Ming; Wang, Leng-Fang; Su, Borcherng.

In: Acta Paediatrica Taiwanica, Vol. 44, No. 6, 11.2003, p. 327-331.

Research output: Contribution to journalArticle

Chen, Chung Ming ; Wang, Leng-Fang ; Su, Borcherng. / Maternal Nicotine Effects on Lung Collagen Gene Expression in Newborn Rats. In: Acta Paediatrica Taiwanica. 2003 ; Vol. 44, No. 6. pp. 327-331.
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