Mast cell-Dependent allergic responses are inhibited by ethanolic extract of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) testa

Hong Jhang Chen, Chun Kuang Shih, Hsin Y. Hsu, Wenchang Chiang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase and cytokine secretion in the late phase of the cell. The purpose of this study was to Investigate the effects of adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) testa against β-hexosaminldase release as a marker of degranulation in rat basophilic leukemia (RBL)-2H3 cells. The ethyl acetate fraction from ethanolic extracts of adlay testa (ATE-EtOAc) exhibited potent inhibitory activity that suppressed degranulation from RBL-2H3 cells stimulated by 1 βM A23187. The 20%80% EtOAc/Hex subfractions of ATE-EtOAc significantly inhibited histamine release with a lC50 of 75-100 μg/mL. In addition, the ATE-EtOAc subfractions suppressed interleukin (IL)-4, IL-6, and tumor necrosis factor-a secretion in RBL-2H3 cells, indicating that adlay testa were able to inhibit cytokine secretion. In order to explore the inhibitory mechanism of adlay testa in mast cell degranulation, we examined the activation of intracellular signaling molecules. Adlay testa inhibited the phosphorylation ERK expression. Furthermore, the two major active compounds, 4-hydroxyacetophenone and p-coumaric acid, were isolated from the ATE-EtOAc subfractions. These results suggest that ATE had an inhibitory effect on allergic response via the ERK signaling transduction in RBL-2H3 cells.

Original languageEnglish
Pages (from-to)2596-2601
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number4
DOIs
Publication statusPublished - Feb 24 2010

Fingerprint

Coix
Coix lacryma-jobi
mast cells
testa
Mast Cells
Rats
Leukemia
leukemia
extracts
Cytokines
Allergies
rats
secretion
Phosphorylation
Cell Degranulation
cells
Interleukin-4
Histamine
Histamine Release
cytokines

Keywords

  • Adlay testa
  • Allergy
  • Cytokines
  • Degranulation
  • ERK
  • Histamine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Mast cell-Dependent allergic responses are inhibited by ethanolic extract of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) testa. / Chen, Hong Jhang; Shih, Chun Kuang; Hsu, Hsin Y.; Chiang, Wenchang.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 4, 24.02.2010, p. 2596-2601.

Research output: Contribution to journalArticle

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