Abstract

Background/Aim: Androgen deprivation therapy remains the principal treatment for patients with advanced prostate cancer, though, most patients will eventually develop hormone-refractory prostate cancer (HRPC). Androgen ablation mediated maspin-induction has been identified in cancer patients. However, the role of maspin on the anticancer activity of curcumin derived from turmeric (Curcuma longa) in HRPC cells has not been elucidated. Materials and Methods: The anticancer action of curcumin in hormone-independent prostate cancer cells (DU145, and PC-3) was determined by measures of cell survival rate. The cause of maspin silencing on the anti-tumor abilities of curcumin in PC-3 cells was evaluated by measures of cell survival rate, cell-cycle distribution, and apoptosis signaling analysis. Results: Our present study showed that PC-3 cells (with higher maspin expression) were more sensitive than DU145 cells to curcumin treatment (with lower maspin expression). RNA interference-mediated maspin silencing reduced curcumin sensitivity of PC-3 cells, as evidenced by reduced apoptotic cell death. After exposure to curcumin, maspin-knockdown cells showed lower expression levels of pro-apoptotic proteins, Bad and Bax, as compared with control cells. Conclusion: Μaspin can enhance the sensitivity of HRPC cells to curcumin treatment.

Original languageEnglish
Pages (from-to)863-870
Number of pages8
JournalAnticancer Research
Volume38
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

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Curcumin
Prostatic Neoplasms
Hormones
Curcuma
Androgens
Cell Survival
Survival Rate
SERPIN-B5
Apoptosis Regulatory Proteins
Therapeutics
RNA Interference
Neoplasms
Cell Cycle
Cell Death
Apoptosis

Keywords

  • Curcumin
  • Hormone-refractory prostate cancer
  • Maspin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

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title = "Maspin enhances the anticancer activity of curcumin in hormone-refractory prostate cancer cells",
abstract = "Background/Aim: Androgen deprivation therapy remains the principal treatment for patients with advanced prostate cancer, though, most patients will eventually develop hormone-refractory prostate cancer (HRPC). Androgen ablation mediated maspin-induction has been identified in cancer patients. However, the role of maspin on the anticancer activity of curcumin derived from turmeric (Curcuma longa) in HRPC cells has not been elucidated. Materials and Methods: The anticancer action of curcumin in hormone-independent prostate cancer cells (DU145, and PC-3) was determined by measures of cell survival rate. The cause of maspin silencing on the anti-tumor abilities of curcumin in PC-3 cells was evaluated by measures of cell survival rate, cell-cycle distribution, and apoptosis signaling analysis. Results: Our present study showed that PC-3 cells (with higher maspin expression) were more sensitive than DU145 cells to curcumin treatment (with lower maspin expression). RNA interference-mediated maspin silencing reduced curcumin sensitivity of PC-3 cells, as evidenced by reduced apoptotic cell death. After exposure to curcumin, maspin-knockdown cells showed lower expression levels of pro-apoptotic proteins, Bad and Bax, as compared with control cells. Conclusion: Μaspin can enhance the sensitivity of HRPC cells to curcumin treatment.",
keywords = "Curcumin, Hormone-refractory prostate cancer, Maspin",
author = "Cheng, {Wan Li} and Huang, {Chien Yu} and Tai, {Cheng Jeng} and Chang, {Yu Jia} and Hung, {Chin Sheng}",
year = "2018",
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T1 - Maspin enhances the anticancer activity of curcumin in hormone-refractory prostate cancer cells

AU - Cheng, Wan Li

AU - Huang, Chien Yu

AU - Tai, Cheng Jeng

AU - Chang, Yu Jia

AU - Hung, Chin Sheng

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Background/Aim: Androgen deprivation therapy remains the principal treatment for patients with advanced prostate cancer, though, most patients will eventually develop hormone-refractory prostate cancer (HRPC). Androgen ablation mediated maspin-induction has been identified in cancer patients. However, the role of maspin on the anticancer activity of curcumin derived from turmeric (Curcuma longa) in HRPC cells has not been elucidated. Materials and Methods: The anticancer action of curcumin in hormone-independent prostate cancer cells (DU145, and PC-3) was determined by measures of cell survival rate. The cause of maspin silencing on the anti-tumor abilities of curcumin in PC-3 cells was evaluated by measures of cell survival rate, cell-cycle distribution, and apoptosis signaling analysis. Results: Our present study showed that PC-3 cells (with higher maspin expression) were more sensitive than DU145 cells to curcumin treatment (with lower maspin expression). RNA interference-mediated maspin silencing reduced curcumin sensitivity of PC-3 cells, as evidenced by reduced apoptotic cell death. After exposure to curcumin, maspin-knockdown cells showed lower expression levels of pro-apoptotic proteins, Bad and Bax, as compared with control cells. Conclusion: Μaspin can enhance the sensitivity of HRPC cells to curcumin treatment.

AB - Background/Aim: Androgen deprivation therapy remains the principal treatment for patients with advanced prostate cancer, though, most patients will eventually develop hormone-refractory prostate cancer (HRPC). Androgen ablation mediated maspin-induction has been identified in cancer patients. However, the role of maspin on the anticancer activity of curcumin derived from turmeric (Curcuma longa) in HRPC cells has not been elucidated. Materials and Methods: The anticancer action of curcumin in hormone-independent prostate cancer cells (DU145, and PC-3) was determined by measures of cell survival rate. The cause of maspin silencing on the anti-tumor abilities of curcumin in PC-3 cells was evaluated by measures of cell survival rate, cell-cycle distribution, and apoptosis signaling analysis. Results: Our present study showed that PC-3 cells (with higher maspin expression) were more sensitive than DU145 cells to curcumin treatment (with lower maspin expression). RNA interference-mediated maspin silencing reduced curcumin sensitivity of PC-3 cells, as evidenced by reduced apoptotic cell death. After exposure to curcumin, maspin-knockdown cells showed lower expression levels of pro-apoptotic proteins, Bad and Bax, as compared with control cells. Conclusion: Μaspin can enhance the sensitivity of HRPC cells to curcumin treatment.

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