Maresin 1 biosynthesis and proresolving anti-infective functions with human-localized aggressive periodontitis leukocytes

Chin Wei Wang, Romain A. Colas, Jesmond Dalli, Hildur H. Arnardottir, Daniel Nguyen, Hatice Hasturk, Nan Chiang, Thomas E. Van Dyke, Charles N. Serhan

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Localized aggressive periodontitis (LAP) is a distinct form of early-onset periodontitis linked to periodontal infection with uncontrolled inflammation and leukocyte-mediated tissue destruction. The resolution of inflammation is an active process orchestrated by specialized proresolving lipid mediators (SPMs). Since the level of the Maresin pathway marker 14-hydroxy-docosahexaenoic acid (14-HDHA) was lower in activated peripheral blood from LAP patients, we investigated the Maresin 1 (MaR1) biosynthetic pathway in these subjects and its role in regulating phagocyte functions. Macrophages from LAP patients had a lower level of expression of 12-lipoxygenase (~30%) and reduced MaR1 (LAP versus healthy controls [HC], 87.8 ± 50 pg/106 cells versus 239.1 ± 32 pg/106 cells). Phagocytosis by LAP macrophages was reduced ~40% compared to that of HC, and killing of periodontal pathogens, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, were similarly reduced. LAP neutrophils also displayed slower kinetics (~30%) and decreased maximal phagocytosis (~20% lower) with these pathogens than those of HC. The administration of MaR1 at 1 nM enhanced phagocytosis (31 to 65% increase), intracellular antimicrobial reactive oxygen species production (26 to 71% increase), bacterial killing of these periodontal pathogens (22 to 38% reduction of bacterial titers), and restored impairment of LAP phagocytes. Together, these results suggest that therapeutics targeting the Maresin pathway have clinical utility in treating LAP and other oral diseases associated with infection, inflammation, and altered phagocyte functions.

Original languageEnglish
Pages (from-to)658-665
Number of pages8
JournalInfection and Immunity
Volume84
Issue number3
DOIs
Publication statusPublished - Mar 2016
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Maresin 1 biosynthesis and proresolving anti-infective functions with human-localized aggressive periodontitis leukocytes'. Together they form a unique fingerprint.

Cite this