Marchantin H as a natural antioxidant and free radical scavenger

George Hsiao, Che Ming Teng, Chia Li Wu, Feng Nien Ko

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The antioxidant activity of marchantin H was investigated using various experimental models. Marchantin H inhibited nonenzymatic iron-induced lipid peroxidation in rat brain homogenates with an IC50 value of 0.51 ± 0.03 μM. It was more potent than desferrioxamine or other classical antioxidants. Marchantin H also suppressed NADPH-dependent microsomal lipid peroxidation with an IC50 value of 0.32 ± 0.01 μM without affecting microsomal electron transport of NADPH-cytochrome P450 reductase. Marchantin H could scavenge the stable free radical 1,1-diphenyl-2-picrylhydrazyl and peroxyl radical derived from 2,2'-azobis(2-amidinopropane) dihydrochloride in aqueous phase, but not the peroxyl radical derived from 2,2'-azobis(2,4- dimethylvaleronitrile) in hexane. The oxygen consumption during peroxyl radical-induced human erythrocyte ghost oxidation was decreased in a concentration-dependent manner by marchantin H. Furthermore, it was reactive toward superoxide anion generated by the xanthine/xanthine oxidase system. On the other hand, marchantin H inhibited copper-catalyzed oxidation of human low-density lipoprotein, as measured by fluorescence intensity, thiobarbituric acid-reactive substance formation, and electrophoretic mobility in a concentration-dependent manner. Our results indicate that marchantin H is a potentially effective and versatile antioxidant and can be used as a chaperone protecting biomacromolecules against peroxidative damage.

Original languageEnglish
Pages (from-to)18-26
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume334
Issue number1
DOIs
Publication statusPublished - Oct 1 1996
Externally publishedYes

Fingerprint

Free Radical Scavengers
Antioxidants
Lipid Peroxidation
Inhibitory Concentration 50
Lipids
Oxidation
NADPH-Ferrihemoprotein Reductase
Electrophoretic mobility
Xanthine
Deferoxamine
Thiobarbituric Acid Reactive Substances
Xanthine Oxidase
Erythrocyte Membrane
Hexanes
Electron Transport
marchantin H
NADP
LDL Lipoproteins
Oxygen Consumption
Superoxides

Keywords

  • antioxidant
  • free radical
  • lipid peroxidation
  • liver microsome
  • low-density lipoprotein
  • marchantin H
  • peroxyl radical
  • superoxide anion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Marchantin H as a natural antioxidant and free radical scavenger. / Hsiao, George; Teng, Che Ming; Wu, Chia Li; Ko, Feng Nien.

In: Archives of Biochemistry and Biophysics, Vol. 334, No. 1, 01.10.1996, p. 18-26.

Research output: Contribution to journalArticle

Hsiao, George ; Teng, Che Ming ; Wu, Chia Li ; Ko, Feng Nien. / Marchantin H as a natural antioxidant and free radical scavenger. In: Archives of Biochemistry and Biophysics. 1996 ; Vol. 334, No. 1. pp. 18-26.
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