MAPK-dependent regulation of IL-1-and β-adrenoreceptor-induced inflammatory cytokine production from mast cells

Implications for the stress response

David S. Chi, S. Matthew Fitzgerald, Shannon Pitts, Karen Cantor, Ellis King, Steve A. Lee, Shau Ku Huang, Guha Krishnaswamy

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: Catecholamines, such as epinephrine, are elaborated in stress responses, and mediate vasoconstriction to cause elevation in systemic vascular resistance and blood pressure. Our previous study has shown that IL-1 can induce mast cells to produce proinflammatory cytokines which are involved in atherogenesis. The aim of this study was to determine the effects of epinephrine on IL-1-induced proatherogenic cytokine production from mast cells. Results: Two ml of HMC-1 (0.75 × 106 cells/ml) were cultured with epinephrine (1 × 10-5 M) in the presence or absence of IL-1β (10 ng/ml) for 24 hrs. HMC-1 cultured alone produced none to trace amounts of IL-6, IL-8, and IL-13. IL-1β significantly induced production of these cytokines in HMC-1, while epinephrine alone did not. However, IL-6, IL-8, and IL-13 production induced by IL-1β were significantly enhanced by addition of epinephrine. The enhancing effect appears to involve NF-κB and p38 MAPK pathways. Flow cytometry showed the presence of β1 and β2 adrenoreceptors on resting mast cells. The enhancing effect of proatherogenic cytokine production by epinephrine was down regulated by the β1 and β2 adrenoceptor antagonist, propranolol, but not by the β1 adrenoceptor antagonist, atenolol, suggesting the effect involved β2 adrenoceptors. The enhancing effect of epinephrine on proatherogenic cytokine production was also down regulated by the immunosuppressive drug, dexamethasone. Conclusions: These results not only confirm that an acute phase cytokine, IL-1β, regulates mast cell function, but also show that epinephrine up regulates the IL-1β induction of proatherogenic cytokines in mast cells. These data provide a novel role for epinephrine, a stress hormone, in inflammation and atherogenesis.

Original languageEnglish
Article number22
JournalBMC Immunology
Volume5
DOIs
Publication statusPublished - Sep 21 2004
Externally publishedYes

Fingerprint

Interleukin-1
Mast Cells
Epinephrine
Cytokines
Adrenergic Receptors
Interleukin-13
Interleukin-8
Interleukin-6
Atherosclerosis
Atenolol
p38 Mitogen-Activated Protein Kinases
Immunosuppressive Agents
Vasoconstriction
Propranolol
Vascular Resistance
Interleukin-10
Dexamethasone
Catecholamines
Flow Cytometry
Up-Regulation

ASJC Scopus subject areas

  • Immunology

Cite this

MAPK-dependent regulation of IL-1-and β-adrenoreceptor-induced inflammatory cytokine production from mast cells : Implications for the stress response. / Chi, David S.; Fitzgerald, S. Matthew; Pitts, Shannon; Cantor, Karen; King, Ellis; Lee, Steve A.; Huang, Shau Ku; Krishnaswamy, Guha.

In: BMC Immunology, Vol. 5, 22, 21.09.2004.

Research output: Contribution to journalArticle

Chi, David S. ; Fitzgerald, S. Matthew ; Pitts, Shannon ; Cantor, Karen ; King, Ellis ; Lee, Steve A. ; Huang, Shau Ku ; Krishnaswamy, Guha. / MAPK-dependent regulation of IL-1-and β-adrenoreceptor-induced inflammatory cytokine production from mast cells : Implications for the stress response. In: BMC Immunology. 2004 ; Vol. 5.
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abstract = "Background: Catecholamines, such as epinephrine, are elaborated in stress responses, and mediate vasoconstriction to cause elevation in systemic vascular resistance and blood pressure. Our previous study has shown that IL-1 can induce mast cells to produce proinflammatory cytokines which are involved in atherogenesis. The aim of this study was to determine the effects of epinephrine on IL-1-induced proatherogenic cytokine production from mast cells. Results: Two ml of HMC-1 (0.75 × 106 cells/ml) were cultured with epinephrine (1 × 10-5 M) in the presence or absence of IL-1β (10 ng/ml) for 24 hrs. HMC-1 cultured alone produced none to trace amounts of IL-6, IL-8, and IL-13. IL-1β significantly induced production of these cytokines in HMC-1, while epinephrine alone did not. However, IL-6, IL-8, and IL-13 production induced by IL-1β were significantly enhanced by addition of epinephrine. The enhancing effect appears to involve NF-κB and p38 MAPK pathways. Flow cytometry showed the presence of β1 and β2 adrenoreceptors on resting mast cells. The enhancing effect of proatherogenic cytokine production by epinephrine was down regulated by the β1 and β2 adrenoceptor antagonist, propranolol, but not by the β1 adrenoceptor antagonist, atenolol, suggesting the effect involved β2 adrenoceptors. The enhancing effect of epinephrine on proatherogenic cytokine production was also down regulated by the immunosuppressive drug, dexamethasone. Conclusions: These results not only confirm that an acute phase cytokine, IL-1β, regulates mast cell function, but also show that epinephrine up regulates the IL-1β induction of proatherogenic cytokines in mast cells. These data provide a novel role for epinephrine, a stress hormone, in inflammation and atherogenesis.",
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