Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity

Suzanne Madgwick, Victoria L. Nixon, Heng Yu Chang, Mary Herbert, Mark Levasseur, Keith T. Jones

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Mammalian eggs naturally arrest at metaphase of the second meiotic division, until sperm triggers a series of Ca 2+ spikes that result in activation of the anaphase-promoting complex/cyclosome (APC/C). APC/C activation at metaphase targets destruction-box containing substrates, such as cyclin B1 and securin, for degradation, and as such eggs complete the second meiotic division. Cyclin B1 degradation reduces maturation (M-phase)-promoting factor (MPF) activity and securin degradation allows sister chromatid separation. Here we examined the second meiotic division in mouse eggs following expression of a cyclin B1 construct with an N-terminal 90 amino acid deletion (Δ90 cyclin B1) that was visualized by coupling to EGFP. This cyclin construct was not an APC/C substrate, and so following fertilization, sperm were incapable of stimulating Δ90 cyclin B1 degradation. In these eggs, chromatin remained condensed and no pronuclei formed. As a consequence of the lack of pronucleus formation, sperm-triggered Ca 2+ spiking continued indefinitely, consistent with a current model in which the sperm-activating factor is localized to the nucleus. Because Ca 2+ spiking was not inhibited by Δ90 cyclin B1, the degradation timing of securin, visualized by coupling it to EGFP, was unaffected. However, despite rapid securin degradation, sister chromatids remained attached. This was a direct consequence of MPF activity because separation was induced following application of the MPF inhibitor roscovitine. Similar observations regarding the ability of MPF to prevent sister chromatid separation have recently been made in Xenopus egg extracts and in HeLa cells. The results presented here show this mechanism can also occur in intact mammalian eggs and further that this mechanism appears conserved among vertebrates. We present a model in which metaphase II arrest is maintained primarily by MPF levels only. Crown

Original languageEnglish
Pages (from-to)68-81
Number of pages14
JournalDevelopmental Biology
Volume275
Issue number1
DOIs
Publication statusPublished - Nov 1 2004
Externally publishedYes

Fingerprint

Maturation-Promoting Factor
Cyclin B1
Chromatids
Securin
Eggs
Maintenance
Anaphase-Promoting Complex-Cyclosome
Spermatozoa
Metaphase
Cyclins
Xenopus
Crowns
HeLa Cells
Fertilization
Chromatin
Ovum
Vertebrates
Amino Acids

Keywords

  • Calcium
  • Cell cycle proteins
  • Chromatids
  • Fertilization
  • Fluorescence
  • Mammals
  • Maturation-promoting factor
  • Meiosis
  • Mice
  • Oocytes

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Madgwick, S., Nixon, V. L., Chang, H. Y., Herbert, M., Levasseur, M., & Jones, K. T. (2004). Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity. Developmental Biology, 275(1), 68-81. https://doi.org/10.1016/j.ydbio.2004.07.024

Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity. / Madgwick, Suzanne; Nixon, Victoria L.; Chang, Heng Yu; Herbert, Mary; Levasseur, Mark; Jones, Keith T.

In: Developmental Biology, Vol. 275, No. 1, 01.11.2004, p. 68-81.

Research output: Contribution to journalArticle

Madgwick, S, Nixon, VL, Chang, HY, Herbert, M, Levasseur, M & Jones, KT 2004, 'Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity', Developmental Biology, vol. 275, no. 1, pp. 68-81. https://doi.org/10.1016/j.ydbio.2004.07.024
Madgwick, Suzanne ; Nixon, Victoria L. ; Chang, Heng Yu ; Herbert, Mary ; Levasseur, Mark ; Jones, Keith T. / Maintenance of sister chromatid attachment in mouse eggs through maturation-promoting factor activity. In: Developmental Biology. 2004 ; Vol. 275, No. 1. pp. 68-81.
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