Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages

Jin Shan Chen, Yuh Lien Chen, Andrew S. Greenberg, Yi Jen Chen, Seu Mei Wang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study investigated the effect of magnolol, a compound isolated from Magnolia officinalis, on lipolysis in lipid-laden RAW 264.7 macrophages. Treatment of macrophages with magnolol led to dissolution of lipid droplets. This phenomenon was accompanied by a dose-dependent release of glycerol and cholesterol and a concomitant reduction in intracellular levels of glycerol and cholesterol. Furthermore, adipose differentiation-related protein (ADRP), a lipid droplet-associated protein, was down-regulated by magnolol in a dose- and time-dependent manner by Western blot analysis. Immunofluorescence studies also showed that ADRP became detached from the surface of lipid droplets after magnolol treatment. The lipolytic effect of magnolol was not mediated through the cAMP-protein kinase A (PKA) system, an authentic lipolytic pathway for macrophages, since magnolol did not induce an increase of intracellular cAMP levels, and pretreatment with either of PKA inhibitors, PKI and KT5720, did not abrogate the lipolytic response to magnolol. We conclude that magnolol induce-lipolysis of lipid-laden macrophages by down-regulation of ADRP expression and detachment of ADRP from the lipid droplet surface by a cAMP-independent mechanism. Lipolysis of lipid-laden macrophages may occur when the amount of ADRP on the surface of lipid droplets is not enough to stabilize the lipid droplets.

Original languageEnglish
Pages (from-to)1028-1037
Number of pages10
JournalJournal of Cellular Biochemistry
Volume94
Issue number5
DOIs
Publication statusPublished - Apr 1 2005

Fingerprint

Macrophages
Lipolysis
Lipids
Proteins
Cyclic AMP-Dependent Protein Kinases
Glycerol
Magnolia
Cholesterol
magnolol
Protein Kinase Inhibitors
Fluorescent Antibody Technique
Down-Regulation
Western Blotting
Lipid Droplets
Perilipin-2
Dissolution
Therapeutics

Keywords

  • ADRP
  • Lipolysis
  • Macrophages
  • Magnonol
  • Signaling pathway

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Chen, J. S., Chen, Y. L., Greenberg, A. S., Chen, Y. J., & Wang, S. M. (2005). Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages. Journal of Cellular Biochemistry, 94(5), 1028-1037. https://doi.org/10.1002/jcb.20361

Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages. / Chen, Jin Shan; Chen, Yuh Lien; Greenberg, Andrew S.; Chen, Yi Jen; Wang, Seu Mei.

In: Journal of Cellular Biochemistry, Vol. 94, No. 5, 01.04.2005, p. 1028-1037.

Research output: Contribution to journalArticle

Chen, JS, Chen, YL, Greenberg, AS, Chen, YJ & Wang, SM 2005, 'Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages', Journal of Cellular Biochemistry, vol. 94, no. 5, pp. 1028-1037. https://doi.org/10.1002/jcb.20361
Chen, Jin Shan ; Chen, Yuh Lien ; Greenberg, Andrew S. ; Chen, Yi Jen ; Wang, Seu Mei. / Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages. In: Journal of Cellular Biochemistry. 2005 ; Vol. 94, No. 5. pp. 1028-1037.
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