Magnolol potently suppressed lipopolysaccharide-induced iNOS and COX-2 expression via downregulating MAPK and NF-κB signaling pathways

Ching Shu Lai, You Syuan Lai, Daih Huang Kuo, Chih Hsiung Wu, Chi Tang Ho, Min Hsiung Pan

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Magnolol is a hydroxylated biphenyl compound from the bark of Magnolia officinalis that has been reported to have various biological properties including anti-inflammation. However, the molecular mechanism of anti-inflammation remains unclear although it has been suggested that magnolol inhibits NO production in murine macrophage. In this study, we investigated the inhibitory effects of magnolol on the induction of NO synthase (NOS) and COX-2 in RAW 264.7 cells induced by lipopolysaccharide (LPS). Co-treatment with magnolol significantly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression. Western blot analysis and reporter assay showed that magnolol reduced translocation of the p50 and p65 subunit by reducing the degradation and phosphorylation of inhibitor κB (IκB), and subsequent transcriptional activity of NF-κB. We also found that magnolol blocked LPS-induced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, Jun N-terminal kinase (JNK) 1/2 and phosphatidylinositiol 3-kinase (PI3. K)/Akt signaling but no p38 mitogen-activated protein kinase (MAPK). These results suggest that magnolol inhibits iNOS and COX-2 protein and gene expression by blocking the activation of NF-κB through interference with activation of PI3K/Akt and MAPK signaling. These findings suggest that magnolol may have potential to be developed into an effective anti-inflammatory agent.

Original languageEnglish
Pages (from-to)198-206
Number of pages9
JournalJournal of Functional Foods
Volume3
Issue number3
DOIs
Publication statusPublished - Jul 2011

Keywords

  • Cyclooxygenase-2 (COX-2)
  • Inducible NO synthase (iNOS)
  • Lipopolysaccharide (LPS)
  • Magnolol
  • Mitogen-activated protein kinase (MAPK)
  • NF-κB
  • Phosphatidylinositol 3-kinase (PI3K)
  • RAW 264.7 monocyte/macrophages

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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