Magnolol nanoparticles exhibit improved water solubility and suppress TNF-α-induced VCAM-1 expression in endothelial cells

Chiang Wen Lee, Stephen Chu Sung Hu, Feng Lin Yen, Lee Fen Hsu, I-Ta Lee, Zih Chan Lin, Ming Horng Tsai, Chieh Liang Huang, Chan Jung Liang, Yao Chang Chiang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The expression of the adhesion molecule vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells enables the attachment of leukocytes to the endothelium, which may lead to inflammation and the development of atherosclerosis. Magnolol is a major bioactive compound derived from the plant species Magnolia officinalis. In this study, we synthesized a novel nanoparticle formulation of magnolol to improve its water solubility and physicochemical properties, evaluated its effects on TNF-α-induced VCAM-1 expression in endothelial cells, and determined the signal transduction pathways involved. Our findings demonstrated that the magnolol nanoparticle system showed great improvements in physicochemical properties and water solubility owing to a reduction in particle size, transformation from a crystalline to amorphous structure, and the formation of hydrogen bonds with the nanoparticle carriers. In terms of its biological actions, magnolol nanoparticles attenuated TNF-α-induced VCAM-1 protein expression, promoter activity, and mRNA expression in endothelial cells in vitro. This was found to be mediated by the ERK, AKT, and NF-κB signaling pathways. In addition, magnolol nanoparticles inhibited TNF-α-induced leukocyte adhesion to endothelial cells, and suppressed TNF-α-induced VCAM-1 expression in the aortic endothelium of mice. In summary, since magnolol nanoparticles inhibit endothelial VCAM-1 expression and leukocyte adhesion to endothelial cells, this novel drug formulation may be a potentially useful therapeutic formulation to prevent the development of atherosclerosis and inflammatory diseases.

Original languageEnglish
Pages (from-to)255-268
Number of pages14
JournalJournal of Biomedical Nanotechnology
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 1 2017
Externally publishedYes

Fingerprint

Vascular Cell Adhesion Molecule-1
Cell adhesion
Endothelial cells
Nanoparticles
Solubility
Endothelial Cells
Molecules
Water
Leukocytes
Adhesion
Endothelium
Atherosclerosis
Magnolia
Signal transduction
Drug Compounding
Phytochemicals
Particle Size
magnolol
Hydrogen
Signal Transduction

Keywords

  • Atherosclerosis
  • Endothelial Cells
  • Inflammation
  • Magnolol
  • Nanoparticles
  • Vascular Cell Adhesion Molecule-1

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Magnolol nanoparticles exhibit improved water solubility and suppress TNF-α-induced VCAM-1 expression in endothelial cells. / Lee, Chiang Wen; Hu, Stephen Chu Sung; Yen, Feng Lin; Hsu, Lee Fen; Lee, I-Ta; Lin, Zih Chan; Tsai, Ming Horng; Huang, Chieh Liang; Liang, Chan Jung; Chiang, Yao Chang.

In: Journal of Biomedical Nanotechnology, Vol. 13, No. 3, 01.03.2017, p. 255-268.

Research output: Contribution to journalArticle

Lee, Chiang Wen ; Hu, Stephen Chu Sung ; Yen, Feng Lin ; Hsu, Lee Fen ; Lee, I-Ta ; Lin, Zih Chan ; Tsai, Ming Horng ; Huang, Chieh Liang ; Liang, Chan Jung ; Chiang, Yao Chang. / Magnolol nanoparticles exhibit improved water solubility and suppress TNF-α-induced VCAM-1 expression in endothelial cells. In: Journal of Biomedical Nanotechnology. 2017 ; Vol. 13, No. 3. pp. 255-268.
@article{a99f5575082f474792ed8c131ce52706,
title = "Magnolol nanoparticles exhibit improved water solubility and suppress TNF-α-induced VCAM-1 expression in endothelial cells",
abstract = "The expression of the adhesion molecule vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells enables the attachment of leukocytes to the endothelium, which may lead to inflammation and the development of atherosclerosis. Magnolol is a major bioactive compound derived from the plant species Magnolia officinalis. In this study, we synthesized a novel nanoparticle formulation of magnolol to improve its water solubility and physicochemical properties, evaluated its effects on TNF-α-induced VCAM-1 expression in endothelial cells, and determined the signal transduction pathways involved. Our findings demonstrated that the magnolol nanoparticle system showed great improvements in physicochemical properties and water solubility owing to a reduction in particle size, transformation from a crystalline to amorphous structure, and the formation of hydrogen bonds with the nanoparticle carriers. In terms of its biological actions, magnolol nanoparticles attenuated TNF-α-induced VCAM-1 protein expression, promoter activity, and mRNA expression in endothelial cells in vitro. This was found to be mediated by the ERK, AKT, and NF-κB signaling pathways. In addition, magnolol nanoparticles inhibited TNF-α-induced leukocyte adhesion to endothelial cells, and suppressed TNF-α-induced VCAM-1 expression in the aortic endothelium of mice. In summary, since magnolol nanoparticles inhibit endothelial VCAM-1 expression and leukocyte adhesion to endothelial cells, this novel drug formulation may be a potentially useful therapeutic formulation to prevent the development of atherosclerosis and inflammatory diseases.",
keywords = "Atherosclerosis, Endothelial Cells, Inflammation, Magnolol, Nanoparticles, Vascular Cell Adhesion Molecule-1",
author = "Lee, {Chiang Wen} and Hu, {Stephen Chu Sung} and Yen, {Feng Lin} and Hsu, {Lee Fen} and I-Ta Lee and Lin, {Zih Chan} and Tsai, {Ming Horng} and Huang, {Chieh Liang} and Liang, {Chan Jung} and Chiang, {Yao Chang}",
year = "2017",
month = "3",
day = "1",
doi = "10.1166/jbn.2017.2342",
language = "English",
volume = "13",
pages = "255--268",
journal = "Journal of Biomedical Nanotechnology",
issn = "1550-7033",
publisher = "American Scientific Publishers",
number = "3",

}

TY - JOUR

T1 - Magnolol nanoparticles exhibit improved water solubility and suppress TNF-α-induced VCAM-1 expression in endothelial cells

AU - Lee, Chiang Wen

AU - Hu, Stephen Chu Sung

AU - Yen, Feng Lin

AU - Hsu, Lee Fen

AU - Lee, I-Ta

AU - Lin, Zih Chan

AU - Tsai, Ming Horng

AU - Huang, Chieh Liang

AU - Liang, Chan Jung

AU - Chiang, Yao Chang

PY - 2017/3/1

Y1 - 2017/3/1

N2 - The expression of the adhesion molecule vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells enables the attachment of leukocytes to the endothelium, which may lead to inflammation and the development of atherosclerosis. Magnolol is a major bioactive compound derived from the plant species Magnolia officinalis. In this study, we synthesized a novel nanoparticle formulation of magnolol to improve its water solubility and physicochemical properties, evaluated its effects on TNF-α-induced VCAM-1 expression in endothelial cells, and determined the signal transduction pathways involved. Our findings demonstrated that the magnolol nanoparticle system showed great improvements in physicochemical properties and water solubility owing to a reduction in particle size, transformation from a crystalline to amorphous structure, and the formation of hydrogen bonds with the nanoparticle carriers. In terms of its biological actions, magnolol nanoparticles attenuated TNF-α-induced VCAM-1 protein expression, promoter activity, and mRNA expression in endothelial cells in vitro. This was found to be mediated by the ERK, AKT, and NF-κB signaling pathways. In addition, magnolol nanoparticles inhibited TNF-α-induced leukocyte adhesion to endothelial cells, and suppressed TNF-α-induced VCAM-1 expression in the aortic endothelium of mice. In summary, since magnolol nanoparticles inhibit endothelial VCAM-1 expression and leukocyte adhesion to endothelial cells, this novel drug formulation may be a potentially useful therapeutic formulation to prevent the development of atherosclerosis and inflammatory diseases.

AB - The expression of the adhesion molecule vascular cell adhesion molecule-1 (VCAM-1) on endothelial cells enables the attachment of leukocytes to the endothelium, which may lead to inflammation and the development of atherosclerosis. Magnolol is a major bioactive compound derived from the plant species Magnolia officinalis. In this study, we synthesized a novel nanoparticle formulation of magnolol to improve its water solubility and physicochemical properties, evaluated its effects on TNF-α-induced VCAM-1 expression in endothelial cells, and determined the signal transduction pathways involved. Our findings demonstrated that the magnolol nanoparticle system showed great improvements in physicochemical properties and water solubility owing to a reduction in particle size, transformation from a crystalline to amorphous structure, and the formation of hydrogen bonds with the nanoparticle carriers. In terms of its biological actions, magnolol nanoparticles attenuated TNF-α-induced VCAM-1 protein expression, promoter activity, and mRNA expression in endothelial cells in vitro. This was found to be mediated by the ERK, AKT, and NF-κB signaling pathways. In addition, magnolol nanoparticles inhibited TNF-α-induced leukocyte adhesion to endothelial cells, and suppressed TNF-α-induced VCAM-1 expression in the aortic endothelium of mice. In summary, since magnolol nanoparticles inhibit endothelial VCAM-1 expression and leukocyte adhesion to endothelial cells, this novel drug formulation may be a potentially useful therapeutic formulation to prevent the development of atherosclerosis and inflammatory diseases.

KW - Atherosclerosis

KW - Endothelial Cells

KW - Inflammation

KW - Magnolol

KW - Nanoparticles

KW - Vascular Cell Adhesion Molecule-1

UR - http://www.scopus.com/inward/record.url?scp=85016011111&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016011111&partnerID=8YFLogxK

U2 - 10.1166/jbn.2017.2342

DO - 10.1166/jbn.2017.2342

M3 - Article

VL - 13

SP - 255

EP - 268

JO - Journal of Biomedical Nanotechnology

JF - Journal of Biomedical Nanotechnology

SN - 1550-7033

IS - 3

ER -