Magnolol-loaded core-shell hydrogel nanoparticles: Drug release, intracellular uptake, and controlled cytotoxicity for the inhibition of migration of vascular smooth muscle cells

Yen Jen Wang, Yin Chih Chien, Chieh Hsi Wu, Dean Mo Liu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Encapsulation and release behavior of a water-insoluble drug, magnolol, using a core-shell polysaccharide-based nanoparticle, manipulating the cellular internalization and controlled cytotoxic effect of magnolol-loaded nanoparticles over the A10 vascular smooth muscle cells (VSMCs) was reported. A magnolol-polyvinylpyrrolidone (PVP) core phase was prepared, followed encapsulating by an amphiphilic carboxymethyl-hexanoyl chitosan (CHC) shell to form a magnolol-loaded core-shell hydrogel nanoparticles (termed magnolol-CHC nanoparticles). The resulting magnolol-CHC nanoparticles were employed for evaluation of drug release and controlled cytotoxic inhibition of VSMCs migration in vitro. A sustained release of the magnolol from the nanoparticles was determined. The magnolol-CHC nanoparticles exhibited outstanding cellular uptake efficiency, and under a cytotoxic evaluation, an increased antiproliferative effect and effective inhibition of VSMC migration as a result of efficient intracellular delivery of the encapsulated magnolol in comparison to free magnolol was achieved. We then envision a potential intracellular medication strategy with improved biological and therapeutic efficacy using the magnolol-CHC nanoparticles illustrated in this work.

Original languageEnglish
Pages (from-to)2339-2349
Number of pages11
JournalMolecular Pharmaceutics
Volume8
Issue number6
DOIs
Publication statusPublished - Dec 5 2011
Externally publishedYes

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Keywords

  • cell migration inhibition
  • cell uptake
  • core-shell hydrogel nanoparticle
  • intracellular delivery
  • magnolol
  • magnolol-CHC nanoparticle

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Medicine
  • Drug Discovery

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