Abstract

Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.

Original languageEnglish
Pages (from-to)7331-7337
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number16
DOIs
Publication statusPublished - 2009

Fingerprint

Cell proliferation
Glioblastoma
cell proliferation
Up-Regulation
Cell Proliferation
Cells
cyclins
Magnolia
cell lines
Cyclin D3
Cyclin-Dependent Kinase 2
Cyclin B
Cyclin A
Cell Line
Cell Cycle Resting Phase
cyclin-dependent kinase
Oligodeoxyribonucleotides
DNA
Cyclin D1
DNA fragmentation

Keywords

  • CDK2
  • G0/G1 arrest
  • Magnolol
  • P21/Cip1
  • U373 glioblastoma cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

@article{79b6d693fe9f410a9a03ca8ae4042813,
title = "Magnolol inhibits human glioblastoma cell proliferation through upregulation of p21/Cip1",
abstract = "Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.",
keywords = "CDK2, G0/G1 arrest, Magnolol, P21/Cip1, U373 glioblastoma cells",
author = "Chen, {Li Ching} and Liu, {Yu Chi} and Liang, {Yu Chih} and Ho, {Yuan Soon} and Lee, {Wen Sen}",
year = "2009",
doi = "10.1021/jf901477g",
language = "English",
volume = "57",
pages = "7331--7337",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "16",

}

TY - JOUR

T1 - Magnolol inhibits human glioblastoma cell proliferation through upregulation of p21/Cip1

AU - Chen, Li Ching

AU - Liu, Yu Chi

AU - Liang, Yu Chih

AU - Ho, Yuan Soon

AU - Lee, Wen Sen

PY - 2009

Y1 - 2009

N2 - Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.

AB - Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.

KW - CDK2

KW - G0/G1 arrest

KW - Magnolol

KW - P21/Cip1

KW - U373 glioblastoma cells

UR - http://www.scopus.com/inward/record.url?scp=70349210557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349210557&partnerID=8YFLogxK

U2 - 10.1021/jf901477g

DO - 10.1021/jf901477g

M3 - Article

C2 - 19645506

AN - SCOPUS:70349210557

VL - 57

SP - 7331

EP - 7337

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 16

ER -