Abstract
Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.
| Original language | English |
|---|---|
| Pages (from-to) | 7331-7337 |
| Number of pages | 7 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 57 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- CDK2
- G0/G1 arrest
- Magnolol
- P21/Cip1
- U373 glioblastoma cells
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Chemistry(all)
Cite this
Magnolol inhibits human glioblastoma cell proliferation through upregulation of p21/Cip1. / Chen, Li Ching; Liu, Yu Chi; Liang, Yu Chih; Ho, Yuan Soon; Lee, Wen Sen.
In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 16, 2009, p. 7331-7337.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Magnolol inhibits human glioblastoma cell proliferation through upregulation of p21/Cip1
AU - Chen, Li Ching
AU - Liu, Yu Chi
AU - Liang, Yu Chih
AU - Ho, Yuan Soon
AU - Lee, Wen Sen
PY - 2009
Y1 - 2009
N2 - Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.
AB - Previously, we demonstrated that magnolol isolated from the bark of Magnolia officinalis has anticancer activity in colon, hepatoma, and leukemia cell lines. In this study, we show that magnolol concentration dependently (0-40 μM) decreased the cell number in a cultured human glioblastoma cancer cell line (U373) and arrested the cells at the G0/G1 phase of the cell cycle. Magnolol treatment decreased the protein levels of cyclins A and D1 and increased p21/Cip1, but not cyclins B and D3, cyclin-dependent kinase (CDK)2, CDK4, CDC25C, Weel, p27/Kip1, and p53. The CDK2p21/Cip1 complex was increased, and the CDK2 kinase activity was decreased in the magnololtreated U373. Pretreatment of U373 with p21/Cip1 specific antisense oligodeoxynucleotide prevented the magnolol-induced increase of p21/Cip1 protein levels and the decrease of DNA synthesis. Magnolol at a concentration of 100μM induced DNA fragmentation in U373. Our findings suggest the potential applications of magnolol in the treatment of human brain cancers.
KW - CDK2
KW - G0/G1 arrest
KW - Magnolol
KW - P21/Cip1
KW - U373 glioblastoma cells
UR - http://www.scopus.com/inward/record.url?scp=70349210557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349210557&partnerID=8YFLogxK
U2 - 10.1021/jf901477g
DO - 10.1021/jf901477g
M3 - Article
C2 - 19645506
AN - SCOPUS:70349210557
VL - 57
SP - 7331
EP - 7337
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 16
ER -