Magnolol induces apoptosis and inhibits ERK-modulated metastatic potential in hepatocellular carcinoma cells

Lin Yen Kuan, Wei Lung Chen, Jiann Hwa Chen, Fei Ting Hsu, Tsu Te Liu, Wei Ting Chen, Kai Lee Wang, Wen Chang Chen, Yu Chang Liu, Wei Shu Wang

Research output: Contribution to journalArticle

Abstract

Background/Aim: The aim of the present study was to evaluate the anti-cancer effect of magnolol in hepatocellular carcinoma (HCC) cells in vitro. Materials and Methods: HCC SK-Hep1 cells were treated with different concentrations of magnolol or PD98059 [extracellular-signal-regulated kinase (ERK) inhibitor] for 48 h, and then cell viability, apoptosis, signal transduction, expression of anti-apoptotic and metastasis-related proteins, and cell invasion were investigated by [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay, flow cytometry, nuclear factor kappa B (NF-κB) reporter gene, western blotting, and cell invasion assays. Results: Magnolol significantly induced accumulation of sub-G1 phase and caspase-3 activation and inhibited NF-κB activation, cell invasion, expression of phosphorylated ERK (pERK), anti-apoptotic and metastatic-related proteins. ERK inactivation was required for magnolol-induced inhibition of metastatic potential of SK-Hep1 cells. Conclusion: Taken together, these results indicated that magnolol not only induced apoptosis, but also inhibited ERK-modulated metastatic potential of HCC SK-Hep1 cells.

Original languageEnglish
Pages (from-to)1361-1368
Number of pages8
JournalIn Vivo
Volume32
Issue number6
DOIs
Publication statusPublished - Nov 1 2018

Fingerprint

Extracellular Signal-Regulated MAP Kinases
Hepatocellular Carcinoma
Cells
Apoptosis
NF-kappa B
Assays
Chemical activation
Signal transduction
Flow cytometry
Caspase 3
Proteins
G1 Phase
Genes
magnolol
Reporter Genes
Signal Transduction
Cell Survival
Flow Cytometry
Western Blotting
Neoplasm Metastasis

Keywords

  • Apoptosis
  • Extracellular-signal-regulated kinase
  • Hepatocellular carcinoma
  • Magnolol

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Kuan, L. Y., Chen, W. L., Chen, J. H., Hsu, F. T., Liu, T. T., Chen, W. T., ... Wang, W. S. (2018). Magnolol induces apoptosis and inhibits ERK-modulated metastatic potential in hepatocellular carcinoma cells. In Vivo, 32(6), 1361-1368. https://doi.org/10.21873/invivo.11387

Magnolol induces apoptosis and inhibits ERK-modulated metastatic potential in hepatocellular carcinoma cells. / Kuan, Lin Yen; Chen, Wei Lung; Chen, Jiann Hwa; Hsu, Fei Ting; Liu, Tsu Te; Chen, Wei Ting; Wang, Kai Lee; Chen, Wen Chang; Liu, Yu Chang; Wang, Wei Shu.

In: In Vivo, Vol. 32, No. 6, 01.11.2018, p. 1361-1368.

Research output: Contribution to journalArticle

Kuan, LY, Chen, WL, Chen, JH, Hsu, FT, Liu, TT, Chen, WT, Wang, KL, Chen, WC, Liu, YC & Wang, WS 2018, 'Magnolol induces apoptosis and inhibits ERK-modulated metastatic potential in hepatocellular carcinoma cells', In Vivo, vol. 32, no. 6, pp. 1361-1368. https://doi.org/10.21873/invivo.11387
Kuan, Lin Yen ; Chen, Wei Lung ; Chen, Jiann Hwa ; Hsu, Fei Ting ; Liu, Tsu Te ; Chen, Wei Ting ; Wang, Kai Lee ; Chen, Wen Chang ; Liu, Yu Chang ; Wang, Wei Shu. / Magnolol induces apoptosis and inhibits ERK-modulated metastatic potential in hepatocellular carcinoma cells. In: In Vivo. 2018 ; Vol. 32, No. 6. pp. 1361-1368.
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