Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis

Hsiao Chien Chen, Jian Tai Qiu, Fu Liang Yang, Yin Chih Liu, Min Cheng Chen, Rung Ywan Tsai, Hung Wei Yang, Chia Yi Lin, Chu Chi Lin, Tzong Shoon Wu, Yi Ming Tu, Min Cong Xiao, Chia Hua Ho, Chien Chao Huang, Chao Sung Lai, Mu Yi Hua

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study proposes a vascular endothelial growth factor (VEGF) biosensor for diagnosing various stages of cervical carcinoma. In addition, VEGF concentrations at various stages of cancer therapy are determined and compared to data obtained by computed tomography (CT) and cancer antigen 125 (CA-125). The increase in VEGF concentrations during operations offers useful insight into dosage timing during cancer therapy. This biosensor uses Avastin as the biorecognition element for the potential cancer biomarker VEGF and is based on a n-type polycrystalline silicon nanowire field-effect transistor (poly-SiNW-FET). Magnetic nanoparticles with poly[aniline-co-N-(1-one-butyric acid) aniline]-Fe3O4 (SPAnH-Fe3O4) shell-core structures are used as carriers for Avastin loading and provide rapid purification due to their magnetic properties, which prevent the loss of bioactivity; furthermore, the high surface area of these structures increases the quantity of Avastin immobilized. Average concentrations in human blood for species that interfere with detection specificity are also evaluated. The detection range of the biosensor for serum samples covers the results expected from both healthy individuals and cancer patients.

Original languageEnglish
Pages (from-to)9443-9450
Number of pages8
JournalAnalytical Chemistry
Volume86
Issue number19
DOIs
Publication statusPublished - Oct 7 2014
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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  • Cite this

    Chen, H. C., Qiu, J. T., Yang, F. L., Liu, Y. C., Chen, M. C., Tsai, R. Y., Yang, H. W., Lin, C. Y., Lin, C. C., Wu, T. S., Tu, Y. M., Xiao, M. C., Ho, C. H., Huang, C. C., Lai, C. S., & Hua, M. Y. (2014). Magnetic-Composite-Modified Polycrystalline Silicon Nanowire Field-Effect Transistor for Vascular Endothelial Growth Factor Detection and Cancer Diagnosis. Analytical Chemistry, 86(19), 9443-9450. https://doi.org/10.1021/ac5001898