Magnesium sulfate mitigates lung injury induced by bilateral lower limb ischemia-reperfusion in rats

Ming Chang Kao, Woan Ching Jan, Pei Shan Tsai, Tao Yeuan Wang, Chun Jen Huang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Lower limb ischemia-reperfusion (I/R) elicits oxidative stress and causes inflammation in lung tissues that may lead to lung injury. Magnesium sulfate (MgSO 4) possesses potent anti-oxidation and anti-inflammation capacity. We sought to elucidate whether MgSO 4 could mitigate I/R-induced lung injury. As MgSO 4 is an L-type calcium channel inhibitor, the role of the L-type calcium channels was elucidated. Materials and Methods: Adult male rats were allocated to receive I/R, I/R plus MgSO 4 (10, 50, or 100 mg/kg), or I/R plus MgSO 4 (100 mg/kg) plus the L-type calcium channels activator BAY-K8644 (20 μg/kg) (n = 12 in each group). Control groups were run simultaneously. I/R was induced by applying rubber band tourniquets high around each thigh for 3 h followed by reperfusion for 3 h. After euthanization, degrees of lung injury, oxidative stress, and inflammation were determined. Results: Arterial blood gas and histologic assays, including histopathology, leukocyte infiltration (polymorphonuclear leukocytes/alveoli ratio and myeloperoxidase activity), and lung water content, confirmed that I/R caused significant lung injury. Significant increases in inflammatory molecules (chemokine, cytokine, and prostaglandin E 2 concentrations) and lipid peroxidation (malondialdehyde concentration) confirmed that I/R caused significant inflammation and oxidative stress in rat lungs. MgSO 4, at the dosages of 50 and 100 mg/kg but not 10 mg/kg, attenuated the oxidative stress, inflammation, and lung injury induced by I/R. Moreover, BAY-K8644 reversed the protective effects of MgSO 4. Conclusions: MgSO 4 mitigates lung injury induced by bilateral lower limb I/R in rats. The mechanisms may involve inhibiting the L-type calcium channels.

Original languageEnglish
JournalJournal of Surgical Research
Volume171
Issue number1
DOIs
Publication statusPublished - Nov 2011

Fingerprint

Magnesium Sulfate
Lung Injury
Reperfusion
Lower Extremity
Ischemia
L-Type Calcium Channels
Oxidative Stress
Inflammation
Calcium Channel Agonists
Lung
Tourniquets
Rubber
Thigh
Prostaglandins E
Malondialdehyde
Chemokines
Lipid Peroxidation
Peroxidase
Pneumonia
Neutrophils

Keywords

  • IL-6
  • L-type calcium channels
  • MDA
  • MIP-2
  • NO

ASJC Scopus subject areas

  • Surgery

Cite this

Magnesium sulfate mitigates lung injury induced by bilateral lower limb ischemia-reperfusion in rats. / Kao, Ming Chang; Jan, Woan Ching; Tsai, Pei Shan; Wang, Tao Yeuan; Huang, Chun Jen.

In: Journal of Surgical Research, Vol. 171, No. 1, 11.2011.

Research output: Contribution to journalArticle

Kao, Ming Chang ; Jan, Woan Ching ; Tsai, Pei Shan ; Wang, Tao Yeuan ; Huang, Chun Jen. / Magnesium sulfate mitigates lung injury induced by bilateral lower limb ischemia-reperfusion in rats. In: Journal of Surgical Research. 2011 ; Vol. 171, No. 1.
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abstract = "Background: Lower limb ischemia-reperfusion (I/R) elicits oxidative stress and causes inflammation in lung tissues that may lead to lung injury. Magnesium sulfate (MgSO 4) possesses potent anti-oxidation and anti-inflammation capacity. We sought to elucidate whether MgSO 4 could mitigate I/R-induced lung injury. As MgSO 4 is an L-type calcium channel inhibitor, the role of the L-type calcium channels was elucidated. Materials and Methods: Adult male rats were allocated to receive I/R, I/R plus MgSO 4 (10, 50, or 100 mg/kg), or I/R plus MgSO 4 (100 mg/kg) plus the L-type calcium channels activator BAY-K8644 (20 μg/kg) (n = 12 in each group). Control groups were run simultaneously. I/R was induced by applying rubber band tourniquets high around each thigh for 3 h followed by reperfusion for 3 h. After euthanization, degrees of lung injury, oxidative stress, and inflammation were determined. Results: Arterial blood gas and histologic assays, including histopathology, leukocyte infiltration (polymorphonuclear leukocytes/alveoli ratio and myeloperoxidase activity), and lung water content, confirmed that I/R caused significant lung injury. Significant increases in inflammatory molecules (chemokine, cytokine, and prostaglandin E 2 concentrations) and lipid peroxidation (malondialdehyde concentration) confirmed that I/R caused significant inflammation and oxidative stress in rat lungs. MgSO 4, at the dosages of 50 and 100 mg/kg but not 10 mg/kg, attenuated the oxidative stress, inflammation, and lung injury induced by I/R. Moreover, BAY-K8644 reversed the protective effects of MgSO 4. Conclusions: MgSO 4 mitigates lung injury induced by bilateral lower limb I/R in rats. The mechanisms may involve inhibiting the L-type calcium channels.",
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