Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14

Ya Ying Chang, Tzu Yu Lin, Ming Chang Kao, Tsung Ying Chen, Ching Feng Cheng, Chih Shung Wong, Chun Jen Huang

Research output: Contribution to journalArticle

Abstract

We investigated effects of magnesium sulfate (MgSO4) on modulating lipopolysaccharide (LPS)–macrophage binding and cluster of differentiation 14 (CD14) expression. Flow cytometry data revealed that the mean levels of LPS-macrophage binding and membrane-bound CD14 expression (mCD14) in differentiated THP-1 cells (a human monocytic cell line) treated with LPS plus MgSO4 (the LPS + M group) decreased by 28.2% and 25.3% compared with those THP-1 cells treated with LPS only (the LPS group) (P < 0.001 and P = 0.037), indicating that MgSO4 significantly inhibits LPS–macrophage binding and mCD14 expression. Notably, these effects of MgSO4 were counteracted by L-type calcium channel activation. Moreover, the mean level of soluble CD14 (sCD14; proteolytic cleavage product of CD14) in the LPS + M group was 25.6% higher than in the LPS group (P < 0.001), indicating that MgSO4 significantly enhances CD14 proteolytic cleavage. Of note, serine protease inhibition mitigated effects of MgSO4 on both decreasing mCD14 and increasing sCD14. In conclusion, MgSO4 inhibits LPS–macrophage binding through reducing CD14 expression. The mechanisms may involve antagonizing L-type calcium channels and activating serine proteases.

Original languageEnglish
JournalInflammopharmacology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Magnesium Sulfate
Lipopolysaccharides
L-Type Calcium Channels
Serine Proteases
Membranes
Macrophages
Flow Cytometry
Cell Line

Keywords

  • Calcium
  • CD14
  • L-type calcium channels
  • MgSO
  • Protease

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Pharmacology (medical)

Cite this

Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14. / Chang, Ya Ying; Lin, Tzu Yu; Kao, Ming Chang; Chen, Tsung Ying; Cheng, Ching Feng; Wong, Chih Shung; Huang, Chun Jen.

In: Inflammopharmacology, 01.01.2019.

Research output: Contribution to journalArticle

Chang, Ya Ying ; Lin, Tzu Yu ; Kao, Ming Chang ; Chen, Tsung Ying ; Cheng, Ching Feng ; Wong, Chih Shung ; Huang, Chun Jen. / Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14. In: Inflammopharmacology. 2019.
@article{f6b86959aa8e485f80ea6c58b0da08b5,
title = "Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14",
abstract = "We investigated effects of magnesium sulfate (MgSO4) on modulating lipopolysaccharide (LPS)–macrophage binding and cluster of differentiation 14 (CD14) expression. Flow cytometry data revealed that the mean levels of LPS-macrophage binding and membrane-bound CD14 expression (mCD14) in differentiated THP-1 cells (a human monocytic cell line) treated with LPS plus MgSO4 (the LPS + M group) decreased by 28.2{\%} and 25.3{\%} compared with those THP-1 cells treated with LPS only (the LPS group) (P < 0.001 and P = 0.037), indicating that MgSO4 significantly inhibits LPS–macrophage binding and mCD14 expression. Notably, these effects of MgSO4 were counteracted by L-type calcium channel activation. Moreover, the mean level of soluble CD14 (sCD14; proteolytic cleavage product of CD14) in the LPS + M group was 25.6{\%} higher than in the LPS group (P < 0.001), indicating that MgSO4 significantly enhances CD14 proteolytic cleavage. Of note, serine protease inhibition mitigated effects of MgSO4 on both decreasing mCD14 and increasing sCD14. In conclusion, MgSO4 inhibits LPS–macrophage binding through reducing CD14 expression. The mechanisms may involve antagonizing L-type calcium channels and activating serine proteases.",
keywords = "Calcium, CD14, L-type calcium channels, MgSO, Protease",
author = "Chang, {Ya Ying} and Lin, {Tzu Yu} and Kao, {Ming Chang} and Chen, {Tsung Ying} and Cheng, {Ching Feng} and Wong, {Chih Shung} and Huang, {Chun Jen}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s10787-019-00568-7",
language = "English",
journal = "Inflammopharmacology",
issn = "0925-4692",
publisher = "Birkhauser Verlag Basel",

}

TY - JOUR

T1 - Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14

AU - Chang, Ya Ying

AU - Lin, Tzu Yu

AU - Kao, Ming Chang

AU - Chen, Tsung Ying

AU - Cheng, Ching Feng

AU - Wong, Chih Shung

AU - Huang, Chun Jen

PY - 2019/1/1

Y1 - 2019/1/1

N2 - We investigated effects of magnesium sulfate (MgSO4) on modulating lipopolysaccharide (LPS)–macrophage binding and cluster of differentiation 14 (CD14) expression. Flow cytometry data revealed that the mean levels of LPS-macrophage binding and membrane-bound CD14 expression (mCD14) in differentiated THP-1 cells (a human monocytic cell line) treated with LPS plus MgSO4 (the LPS + M group) decreased by 28.2% and 25.3% compared with those THP-1 cells treated with LPS only (the LPS group) (P < 0.001 and P = 0.037), indicating that MgSO4 significantly inhibits LPS–macrophage binding and mCD14 expression. Notably, these effects of MgSO4 were counteracted by L-type calcium channel activation. Moreover, the mean level of soluble CD14 (sCD14; proteolytic cleavage product of CD14) in the LPS + M group was 25.6% higher than in the LPS group (P < 0.001), indicating that MgSO4 significantly enhances CD14 proteolytic cleavage. Of note, serine protease inhibition mitigated effects of MgSO4 on both decreasing mCD14 and increasing sCD14. In conclusion, MgSO4 inhibits LPS–macrophage binding through reducing CD14 expression. The mechanisms may involve antagonizing L-type calcium channels and activating serine proteases.

AB - We investigated effects of magnesium sulfate (MgSO4) on modulating lipopolysaccharide (LPS)–macrophage binding and cluster of differentiation 14 (CD14) expression. Flow cytometry data revealed that the mean levels of LPS-macrophage binding and membrane-bound CD14 expression (mCD14) in differentiated THP-1 cells (a human monocytic cell line) treated with LPS plus MgSO4 (the LPS + M group) decreased by 28.2% and 25.3% compared with those THP-1 cells treated with LPS only (the LPS group) (P < 0.001 and P = 0.037), indicating that MgSO4 significantly inhibits LPS–macrophage binding and mCD14 expression. Notably, these effects of MgSO4 were counteracted by L-type calcium channel activation. Moreover, the mean level of soluble CD14 (sCD14; proteolytic cleavage product of CD14) in the LPS + M group was 25.6% higher than in the LPS group (P < 0.001), indicating that MgSO4 significantly enhances CD14 proteolytic cleavage. Of note, serine protease inhibition mitigated effects of MgSO4 on both decreasing mCD14 and increasing sCD14. In conclusion, MgSO4 inhibits LPS–macrophage binding through reducing CD14 expression. The mechanisms may involve antagonizing L-type calcium channels and activating serine proteases.

KW - Calcium

KW - CD14

KW - L-type calcium channels

KW - MgSO

KW - Protease

UR - http://www.scopus.com/inward/record.url?scp=85061194369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061194369&partnerID=8YFLogxK

U2 - 10.1007/s10787-019-00568-7

DO - 10.1007/s10787-019-00568-7

M3 - Article

AN - SCOPUS:85061194369

JO - Inflammopharmacology

JF - Inflammopharmacology

SN - 0925-4692

ER -