Magnesium sulfate inhibits binding of lipopolysaccharide to THP-1 cells by reducing expression of cluster of differentiation 14

Ya Ying Chang, Tzu Yu Lin, Ming Chang Kao, Tsung Ying Chen, Ching Feng Cheng, Chih Shung Wong, Chun Jen Huang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We investigated effects of magnesium sulfate (MgSO4) on modulating lipopolysaccharide (LPS)–macrophage binding and cluster of differentiation 14 (CD14) expression. Flow cytometry data revealed that the mean levels of LPS-macrophage binding and membrane-bound CD14 expression (mCD14) in differentiated THP-1 cells (a human monocytic cell line) treated with LPS plus MgSO4 (the LPS + M group) decreased by 28.2% and 25.3% compared with those THP-1 cells treated with LPS only (the LPS group) (P < 0.001 and P = 0.037), indicating that MgSO4 significantly inhibits LPS–macrophage binding and mCD14 expression. Notably, these effects of MgSO4 were counteracted by L-type calcium channel activation. Moreover, the mean level of soluble CD14 (sCD14; proteolytic cleavage product of CD14) in the LPS + M group was 25.6% higher than in the LPS group (P < 0.001), indicating that MgSO4 significantly enhances CD14 proteolytic cleavage. Of note, serine protease inhibition mitigated effects of MgSO4 on both decreasing mCD14 and increasing sCD14. In conclusion, MgSO4 inhibits LPS–macrophage binding through reducing CD14 expression. The mechanisms may involve antagonizing L-type calcium channels and activating serine proteases.

Original languageEnglish
JournalInflammopharmacology
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • Calcium
  • CD14
  • L-type calcium channels
  • MgSO
  • Protease

ASJC Scopus subject areas

  • Immunology
  • Pharmacology
  • Pharmacology (medical)

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