Magnesium Sulfate Inhibits Activator Protein-1 Upregulation in Endotoxin-activated Murine Macrophages

Cay Huyen Chen, Pei Shan Tsai, Chun Jen Huang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: The expression of inflammatory molecules is regulated by the transcription factor activator protein-1 (AP-1), a heterodimeric protein that consists of proteins from various families, including c-Jun and c-Fos. We sought to elucidate whether MgSO4 regulates the activation of AP-1 in endotoxin-activated RAW264.7 cells, a murine macrophage-like cell line. The possible roles of the L-type calcium channels in this process were also elucidated. Materials and Methods: RAW264.7 cells were treated with phosphate buffered saline, MgSO4, lipopolysaccharide (LPS), LPS plus MgSO4 (20 mM), or LPS plus MgSO4 plus the L-type calcium channel activator BAY-K8644 (1 μM). After harvesting, expression of AP-1 was evaluated. Results: LPS induced AP-1 activation based on the fact that the nuclear protein concentrations of AP-1 components, including c-Jun and c-Fos, as well as the AP-1 DNA-binding activity, were significantly increased in LPS-treated RAW264.7 cells. MgSO4, in contrast, significantly inhibited LPS-induced AP-1 activation in activated RAW264.7 cells. Moreover, the effect of MgSO4 on AP-1 was reversed by BAY-K8644. Conclusion: MgSO4 inhibited AP-1 activation in LPS-treated macrophages and the mechanism may involve the L-type calcium channels.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalTzu Chi Medical Journal
Volume22
Issue number4
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Magnesium Sulfate
Transcription Factor AP-1
Endotoxins
Up-Regulation
Macrophages
Lipopolysaccharides
L-Type Calcium Channels
Calcium Channel Agonists
Nuclear Proteins
Proteins
Transcription Factors
Phosphates
Cell Line

Keywords

  • AP-1
  • L-type calcium channels
  • LPS
  • MgSO4

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Magnesium Sulfate Inhibits Activator Protein-1 Upregulation in Endotoxin-activated Murine Macrophages. / Chen, Cay Huyen; Tsai, Pei Shan; Huang, Chun Jen.

In: Tzu Chi Medical Journal, Vol. 22, No. 4, 12.2010, p. 177-183.

Research output: Contribution to journalArticle

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N2 - Objective: The expression of inflammatory molecules is regulated by the transcription factor activator protein-1 (AP-1), a heterodimeric protein that consists of proteins from various families, including c-Jun and c-Fos. We sought to elucidate whether MgSO4 regulates the activation of AP-1 in endotoxin-activated RAW264.7 cells, a murine macrophage-like cell line. The possible roles of the L-type calcium channels in this process were also elucidated. Materials and Methods: RAW264.7 cells were treated with phosphate buffered saline, MgSO4, lipopolysaccharide (LPS), LPS plus MgSO4 (20 mM), or LPS plus MgSO4 plus the L-type calcium channel activator BAY-K8644 (1 μM). After harvesting, expression of AP-1 was evaluated. Results: LPS induced AP-1 activation based on the fact that the nuclear protein concentrations of AP-1 components, including c-Jun and c-Fos, as well as the AP-1 DNA-binding activity, were significantly increased in LPS-treated RAW264.7 cells. MgSO4, in contrast, significantly inhibited LPS-induced AP-1 activation in activated RAW264.7 cells. Moreover, the effect of MgSO4 on AP-1 was reversed by BAY-K8644. Conclusion: MgSO4 inhibited AP-1 activation in LPS-treated macrophages and the mechanism may involve the L-type calcium channels.

AB - Objective: The expression of inflammatory molecules is regulated by the transcription factor activator protein-1 (AP-1), a heterodimeric protein that consists of proteins from various families, including c-Jun and c-Fos. We sought to elucidate whether MgSO4 regulates the activation of AP-1 in endotoxin-activated RAW264.7 cells, a murine macrophage-like cell line. The possible roles of the L-type calcium channels in this process were also elucidated. Materials and Methods: RAW264.7 cells were treated with phosphate buffered saline, MgSO4, lipopolysaccharide (LPS), LPS plus MgSO4 (20 mM), or LPS plus MgSO4 plus the L-type calcium channel activator BAY-K8644 (1 μM). After harvesting, expression of AP-1 was evaluated. Results: LPS induced AP-1 activation based on the fact that the nuclear protein concentrations of AP-1 components, including c-Jun and c-Fos, as well as the AP-1 DNA-binding activity, were significantly increased in LPS-treated RAW264.7 cells. MgSO4, in contrast, significantly inhibited LPS-induced AP-1 activation in activated RAW264.7 cells. Moreover, the effect of MgSO4 on AP-1 was reversed by BAY-K8644. Conclusion: MgSO4 inhibited AP-1 activation in LPS-treated macrophages and the mechanism may involve the L-type calcium channels.

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